Ethyl 5-(2-(2-((4-methoxyphenyl)amino)-2-oxoacetyl)-1H-pyrrol-1-yl)-1-phenyl-1H-pyrazole-4-carboxylate CAS 477709-29-8

Description

CATO supplies Ethyl 5-(2-(2-((4-methoxyphenyl)amino)-2-oxoacetyl)-1H-pyrrol-1-yl)-1-phenyl-1H-pyrazole-4-carboxylate (CAS 477709-29-8), a certified reference material (molecular formula C25H22N4O5, MW 458.47 g/mol) for analytical method development and quality control.

The molecular framework of Ethyl 5-(2-(2-((4-methoxyphenyl)amino)-2-oxoacetyl)-1H-pyrrol-1-yl)-1-phenyl-1H-pyrazole-4-carboxylate (C25H22N4O5) is characterized by a flavonoid core, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

As a certified reference material (CRM), Ethyl 5-(2-(2-((4-methoxyphenyl)amino)-2-oxoacetyl)-1H-pyrrol-1-yl)-1-phenyl-1H-pyrazole-4-carboxylate meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

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