Ethyl (E)-1-(2-cyano-3-(dimethylamino)acryloyl)-3-methyl-1H-pyrazole-4-carboxylate CAS 477713-80-7

Description

Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including Ethyl (E)-1-(2-cyano-3-(dimethylamino)acryloyl)-3-methyl-1H-pyrazole-4-carboxylate.

Ethyl (E)-1-(2-cyano-3-(dimethylamino)acryloyl)-3-methyl-1H-pyrazole-4-carboxylate (CAS 477713-80-7) is a high-purity analytical reference standard with the molecular formula C13H16N4O3 and a molecular weight of 276.29 g/mol.

The molecular framework of Ethyl (E)-1-(2-cyano-3-(dimethylamino)acryloyl)-3-methyl-1H-pyrazole-4-carboxylate (C13H16N4O3) is characterized by a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

CATO manufactures Ethyl (E)-1-(2-cyano-3-(dimethylamino)acryloyl)-3-methyl-1H-pyrazole-4-carboxylate under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.

Physical & Chemical Properties

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