| CAS No. | 478247-25-5 |
| Molecular Formula | C25H25FN4O |
| Molecular Weight | 416.49 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
CATO supplies N'-(Diphenylmethylidene)-2-[4-(2-fluorophenyl)piperazin-1-yl]acetohydrazide (CAS 478247-25-5), a certified reference material (molecular formula C25H25FN4O, MW 416.49 g/mol) for analytical method development and quality control.
Owing to a steroid core, an amide group, a hydroxyl group, an ether linkage, fluorine substitution, N'-(Diphenylmethylidene)-2-[4-(2-fluorophenyl)piperazin-1-yl]acetohydrazide is typically pale yellow to yellow crystalline powder. Its solid-state physicochemical attributes — including a molecular weight of 416.49 g/mol — guide the selection of appropriate sample preparation and analytical techniques. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
As a certified reference material (CRM), N'-(Diphenylmethylidene)-2-[4-(2-fluorophenyl)piperazin-1-yl]acetohydrazide meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
| Molecular Formula | C25H25FN4O |
| Molecular Weight | 416.49 g/mol |
| Appearance | Pale yellow to yellow crystalline powder. (slight halide-like odor) |
| Purity | 97%+ |
| Storage | Store at room temperature, protect from light |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light |
| Degree of Unsaturation (DBE) | 15.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 2025 |
| Halogen Content | F-containing |
| Nitrogen Atoms | 4 |