3-(2-Chlorophenyl)-4-((2-hydroxy-3-methoxybenzylidene)amino)-1H-1,2,4-triazole-5(4H)-thione CAS 478253-84-8
| CAS No. | 478253-84-8 |
| Molecular Formula | C16H13ClN4O2S |
| Molecular Weight | 360.82 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including 3-(2-Chlorophenyl)-4-((2-hydroxy-3-methoxybenzylidene)amino)-1H-1,2,4-triazole-5(4H)-thione.
CATO supplies 3-(2-Chlorophenyl)-4-((2-hydroxy-3-methoxybenzylidene)amino)-1H-1,2,4-triazole-5(4H)-thione (CAS 478253-84-8), a certified reference material (molecular formula C16H13ClN4O2S, MW 360.82 g/mol) for analytical method development and quality control.
The molecular framework of 3-(2-Chlorophenyl)-4-((2-hydroxy-3-methoxybenzylidene)amino)-1H-1,2,4-triazole-5(4H)-thione (C16H13ClN4O2S) is characterized by a nitrogen-containing heterocyclic scaffold, an amide group, a sulfone group, a hydroxyl group, an ether linkage, chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
As a certified reference material (CRM), 3-(2-Chlorophenyl)-4-((2-hydroxy-3-methoxybenzylidene)amino)-1H-1,2,4-triazole-5(4H)-thione meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
Physical & Chemical Properties
| Molecular Formula | C16H13ClN4O2S |
| Molecular Weight | 360.82 g/mol |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening; Contains ester bond; hydrolyzes under strong alkaline conditions |
| Degree of Unsaturation (DBE) | 12.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 2025 |
| Halogen Content | Cl-containing |
| Nitrogen Atoms | 4 |
| Sulfur Atoms | 1 |
Related Chlorophenyl Standards
- 2-{[3-(4-chlorophenyl)-4-oxo-3,4-dihydroquinazolin-2-yl]sulfanyl}acetohydrazide (CAS 133764-45-1)
- 2-{[4-(4-chlorophenyl)-5-(pyridin-4-yl)-4h-1,2,4-triazol-3-yl]sulfanyl}acetohydrazide (CAS 893727-15-6)
- 3-(2-Chlorophenyl)-4-((4-hydroxy-3-methoxybenzylidene)amino)-1H-1,2,4-triazole-5(4H)-thione (CAS 478254-19-2)
- 3-(3-Chlorophenyl)-4-((2-hydroxy-3-methoxybenzylidene)amino)-1H-1,2,4-triazole-5(4H)-thione (CAS 478254-64-7)
- 3-(3-Chlorophenyl)-4-((4-hydroxy-3-methoxybenzylidene)amino)-1H-1,2,4-triazole-5(4H)-thione (CAS 478255-03-7)
- N-(2-(2-(3-chlorophenyl)thiazol-4-yl)ethyl)-5-nitropyridin-2-amine (CAS 861207-09-2)
- N-(4-Chlorophenyl)-2-((3-(2-hydroxyphenyl)-1H-1,2,4-triazol-5-yl)thio)acetamide (CAS 332160-20-0)
- Ethyl 3-(4-(((3-chlorophenyl)amino)methyl)benzamido)propanoate (CAS 1624262-11-8)
Recommended Standards
- 2-(3-(1H-pyrrol-1-yl)thiophene-2-carbonyl)-N-(4-chlorophenyl)hydrazine-1-carboxamide (CAS 478062-85-0)
- 2-(3-(1H-pyrrol-1-yl)thiophene-2-carbonyl)-N-(3-chlorophenyl)hydrazine-1-carboxamide (CAS 478062-86-1)
- 2-(2-(Benzo[d]isoxazol-3-yl)acetyl)-N-(4-chlorophenyl)hydrazine-1-carbothioamide (CAS 860651-57-6)
- 4-(5-Chloro-1,3-dihydrobenzo[de]isochromen-6-yl)-6-(methylsulfinyl)-1,3,5-triazin-2-amine (CAS 959763-01-0)
- 4-Chloro-2-(Sulfanylmethyl)phenol (CAS 118143-41-2)
- tert-Butyl (5S)-5-methyl-1,2,3-oxathiazolidine-3-carboxylate 2-oxide (CAS 1311368-74-7)
- 1-(4-Acetylphenyl)-2,4-dioxo-1,2,3,4-tetrahydro-5-pyrimidinecarbonitrile (CAS 446276-09-1)
- N-(2,3-Dihydro-1,4-benzodioxin-6-yl)morpholine-4-carboxamide (CAS 600147-72-6)
- 9-Butylanthracene (CAS 1498-69-7)
- Ceftazidime Impurity 98 (CAS CATO-C4X-121298)
- N-Desthiobiotin-N-bis(PEG4-NHS ester) (CAS 2353409-61-5)
- (3,4,5-Trimethylpyridin-2-yl)methyl acetate (CAS 848696-98-0)
- 2-(Bromomethyl)-4-(trifluoromethyl)pyridine hydrobromide (CAS 2089334-76-7)
- β-Asp9-Tirzepatide (CAS CATO-CPTD788931)
Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
- Worldwide shipping with full export documentation (HS code guidance available)
- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com