(E)-({6-chloro-2-phenylimidazo[1,2-a]pyridin-3-yl}methylidene)amino thiophene-2-carboxylate CAS 478257-31-7

Description

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

(E)-({6-chloro-2-phenylimidazo[1,2-a]pyridin-3-yl}methylidene)amino thiophene-2-carboxylate (CAS 478257-31-7), molecular formula C19H12ClN3O2S and molecular weight 381.84 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of (E)-({6-chloro-2-phenylimidazo[1,2-a]pyridin-3-yl}methylidene)amino thiophene-2-carboxylate (C19H12ClN3O2S) is characterized by a steroid core, an amide group, a sulfone group, a hydroxyl group, an ether linkage, chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Halogen substitution (F/Cl/Br/I) produces a distinctive isotope pattern in mass spectrometry, a valuable aid for qualitative confirmation.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

Physical & Chemical Properties

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