2-{5-[(1E)-1-{[(4-chlorophenyl)methoxy]imino}ethyl]thiophen-2-yl}acetonitrile CAS 478261-08-4

Description

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

2-{5-[(1E)-1-{[(4-chlorophenyl)methoxy]imino}ethyl]thiophen-2-yl}acetonitrile (CAS 478261-08-4), molecular formula C15H13ClN2OS and molecular weight 304.79 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of 2-{5-[(1E)-1-{[(4-chlorophenyl)methoxy]imino}ethyl]thiophen-2-yl}acetonitrile (C15H13ClN2OS) is characterized by a nitrogen-containing heterocyclic scaffold, an amide group, sulfur-containing functionality, a hydroxyl group, an ether linkage, chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by its high hydrophobicity (logP), which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Halogen substitution (F/Cl/Br/I) produces a distinctive isotope pattern in mass spectrometry, a valuable aid for qualitative confirmation.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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