2-Cyano-N'-[(1Z)-[4-(dimethylamino)phenyl]methylidene]acetohydrazide CAS 4974-48-5

Description

Compared with other compounds in its class, the molecular weight of 230.27 g/mol gives 2-Cyano-N'-[(1Z)-[4-(dimethylamino)phenyl]methylidene]acetohydrazide distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

2-Cyano-N'-[(1Z)-[4-(dimethylamino)phenyl]methylidene]acetohydrazide (CAS 4974-48-5), molecular formula C12H14N4O and molecular weight 230.27 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of 2-Cyano-N'-[(1Z)-[4-(dimethylamino)phenyl]methylidene]acetohydrazide (C12H14N4O) is characterized by a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

Recommended for analytical method development and validation; each product is supplied with a full Certificate of Analysis (COA) ensuring ISO 17034 metrological traceability.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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