2-((2-Hydroxyethyl)amino)-2-oxoacetic acid CAS 5270-73-5

Purity 97%+In StockPharmaceutical Standards
CAS No.5270-73-5
Molecular FormulaC4H7NO4
Molecular Weight133.10 g/mol
CategoryPharmaceutical Standards / API Standards
AvailabilityIn Stock

Chemical Structure

2-((2-Hydroxyethyl)amino)-2-oxoacetic acid CAS 5270-73-5 chemical structure

Description

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

CATO supplies 2-((2-Hydroxyethyl)amino)-2-oxoacetic acid (CAS 5270-73-5), a certified reference material (molecular formula C4H7NO4, MW 133.10 g/mol) for analytical method development and quality control.

The molecular framework of 2-((2-Hydroxyethyl)amino)-2-oxoacetic acid (C4H7NO4) is characterized by a carbohydrate-derived structure, an amide group. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

Molecular FormulaC4H7NO4
Molecular Weight133.10 g/mol
Purity97%+
StorageStore at 2-8°C, protect from light, keep sealed and dry
Solubility (reference)Soluble in methanol, ethanol, DMSO, water
StabilityLight-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening
Degree of Unsaturation (DBE)2.0
CAS Registry Year (est.)1958
Nitrogen Atoms1

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