5-Methyl-1-phenyl-3H-pyridazino[4,5-b]indol-4(5H)-one CAS 52817-91-1

Description

Compared with other compounds in its class, the molecular weight of 275.30 g/mol gives 5-Methyl-1-phenyl-3H-pyridazino[4,5-b]indol-4(5H)-one distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

5-Methyl-1-phenyl-3H-pyridazino[4,5-b]indol-4(5H)-one (CAS 52817-91-1), molecular formula C17H13N3O and molecular weight 275.30 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of 5-Methyl-1-phenyl-3H-pyridazino[4,5-b]indol-4(5H)-one (C17H13N3O) is characterized by a steroid core, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by its high hydrophobicity (logP), which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

As a certified reference material (CRM), 5-Methyl-1-phenyl-3H-pyridazino[4,5-b]indol-4(5H)-one meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

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