5-Methyl-4-nitro-1H-pyrazole-3-carboxylic acid CAS 5334-38-3
| CAS No. | 5334-38-3 |
| Molecular Formula | C5H5N3O4 |
| Molecular Weight | 171.11 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
The occurrence of 5-Methyl-4-nitro-1H-pyrazole-3-carboxylic acid is closely related to a carbohydrate-derived structure, an amide group in its molecular formula, which determines its behaviour during synthesis, purification, or degradation.
CATO supplies 5-Methyl-4-nitro-1H-pyrazole-3-carboxylic acid (CAS 5334-38-3), a certified reference material (molecular formula C5H5N3O4, MW 171.11 g/mol) for analytical method development and quality control.
The molecular framework of 5-Methyl-4-nitro-1H-pyrazole-3-carboxylic acid (C5H5N3O4) is characterized by a carbohydrate-derived structure, an amide group. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
CATO manufactures 5-Methyl-4-nitro-1H-pyrazole-3-carboxylic acid under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.
Physical & Chemical Properties
| Molecular Formula | C5H5N3O4 |
| Molecular Weight | 171.11 g/mol |
| Appearance | Off-white to pale yellow crystalline powder. (faint amine-like odor) |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in methanol, ethanol, DMSO, water |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 5.0 |
| UV Absorption | UV-absorbing |
| CAS Registry Year (est.) | 1958 |
| Nitrogen Atoms | 3 |
Related Methyl Standards
- 1-Methyl-4-nitro-1H-imidazole-2-carboxylic acid (CAS 109012-24-0)
- 2-Methyl-5-nitro-1H-imidazole-4-carboxylic acid (CAS 116273-51-9)
- 1-Methyl-5-nitro-1h-pyrazole-3-carboxylic acid (CAS 1171709-53-7)
- 1-(5-Nitro-2H-tetrazol-2-yl)propan-2-one (CAS 131394-19-9)
- Methyl 4-nitro-1H-pyrazole-5-carboxylate (CAS 1345513-95-2)
- 2-(5-Nitro-1H-imidazol-1-yl)acetic acid (CAS 14766-55-3)
- 1-Methyl-3-nitro-1h-pyrazole-5-carboxylic acid (CAS 177409-39-1)
- Methyl 3-nitro-1H-pyrazole-4-carboxylate (CAS 1798715-16-8)
Recommended Standards
- 5-Hydroxyimino-1,3-dimethyl-1,3-diazinane-2,4,6-trione (CAS 5417-13-0)
- 5-Methyl-4-nitro-1H-pyrazole-3-carboxylic acid (CAS 75103-81-0)
- 5-Carbamoylbarbituric acid (CAS 56032-78-1)
- 5-Methyl-4-nitrofuran-2-carboxylic acid (CAS 326867-81-6)
- Ethyl 4-amino-1-(methoxymethyl)-1H-imidazole-2-carboxylate (CAS 1026411-22-2)
- 2-{8-methylimidazo[1,2-a]pyridin-2-yl}acetic acid (CAS 59128-08-4)
- 5-chloro-2-methoxy-N-methyl-3-sulfamoylbenzamide (CAS 1486433-08-2)
- 6-(Trifluoromethyl)benzo[d]oxazole-2(3H)-thione (CAS 149367-81-7)
- 3-[5-(Hydroxymethyl)-2-furyl]benzoic acid (CAS 679821-90-0)
- N-(3,4-Dimethylphenyl)-2-[4-(5-methyl-1,2,4-oxadiazol-3-yl)-1,3-dioxo-1H,2H,3H,5H,6H,7H,8H,9H-pyrimido[1,6-a]azepin-2-yl]acetamide (CAS 1775354-12-5)
- Empagliflozin impurity 204 (CAS 1800196-43-3)
- 2-((3bS,4aR)-3-(Difluoromethyl)-5,5-difluoro-3b,4,4a,5-tetrahydro-1H-cyclopropa[3,4]cyclopenta[1,2-c]pyrazol-1-yl)acetic acid (CAS 1626337-85-6)
- (2R,3S,5R)-5-(2-Amino-6-oxo-3,6-dihydro-9H-purin-9-yl)-2-(((hydroxy(phosphonooxy)phosphoryl)oxy)methyl)tetrahydrofuran-3-yl 2-(methylamino)benzoate (CAS 127383-33-9)
- Semaglutide impurity 22(OtBu-Ste-γGlu(OtBu)-AEEA-AEEA-OH) (CAS 1118767-16-0)
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