Benzylpenicillin Impurity 66 CAS 59034-29-6
| CAS No. | 59034-29-6 |
| Molecular Formula | C16H17N2O4S |
| Molecular Weight | N/A |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
The occurrence of Benzylpenicillin Impurity 66 is closely related to a flavonoid core, an amide group, a sulfone group, a hydroxyl group, an ether linkage in its molecular formula, which determines its behaviour during synthesis, purification, or degradation.
Benzylpenicillin Impurity 66 (CAS 59034-29-6) is a certified pharmaceutical impurity reference standard with the molecular formula C16H17N2O4SNa and a molecular weight of 356.37 g/mol.
The molecular framework of Benzylpenicillin Impurity 66 (C16H17N2O4SNa) is characterized by a flavonoid core, an amide group, a sulfone group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
This reference standard supports related-substances testing and method development in line with ICH Q3A/Q3B guidance on impurity control, and is suitable for system suitability testing and forced-degradation studies.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C16H17N2O4S Na |
| Molecular Weight | 356.37 g/mol |
| Purity | ≥95% |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 9.5 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1973 |
| Nitrogen Atoms | 2 |
| Sulfur Atoms | 1 |
Related Benzylpenicillin Standards
- Benzylpenicillin Potassium EP impurity E (CAS 11039-68-2)
- Benzylpenicillin Impurity 11 (CAS 118-53-6)
- Benzylpenicillin Impurity 21(Mixture of Diastereomers) (CAS 13057-98-2)
- Benzylpenicillin EP Impurity D (CAS 13093-87-3)
- Benzylpenicillin Impurity 18 (CAS 22801-31-6)
- Benzylpenicillin Impurity 68 (CAS 30200-14-7)
- Benzylpenicillin Potassium EP Impurity I potassium salt (CAS 31447-86-6)
- Benzylpenicillin CP Impurity I (CAS 3264-88-8)
Recommended Standards
- Cefadroxil Impurity 38 (CAS CATO-C4X-122438)
- Cenobamate Impurity 25 (CAS CATO-C4X-241625)
- Cloxacillin Impurity 18 (CAS 25629-50-9)
- (R)-1-(tert-butoxycarbonyl)-2-(3-nitrobenzyl)pyrrolidine-2-carboxylic acid (CAS 959582-68-4)
- N-(2-Hydroxy-5-methoxyphenyl)methanesulfonamide (CAS 123664-87-9)
- Bis(2-chloroethoxy)-d8-methane (CAS 93966-78-0)
- N-{[(2,6-Dimethylanilino)carbonyl]oxy}-N-{(E)-2,2-dimethyl-1-[(2-nitrophenoxy)methyl]propylidene}amine (CAS 478043-12-8)
- (E)-4-(benzo[d][1,3]dioxol-5-yl)-2-(4-methoxystyryl)-5-oxo-5H-indeno[1,2-b]pyridine-3-carbonitrile (CAS 866142-84-9)
- 1-(Benzylsulfonyl)-4-(thiophen-3-yl)piperidine (CAS 1396887-11-8)
- Allo-D-Ile12-Tirzepatide (CAS CATO-CPTD788963)
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