| CAS No. | 65148-48-3 |
| Molecular Formula | C5H4N4O |
| Molecular Weight | 136.11 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Recommended for analytical method development and validation; each product is supplied with a full Certificate of Analysis (COA) ensuring ISO 17034 metrological traceability.
CATO supplies [1,2,4]Triazolo[4,3-b]pyridazin-6-ol (CAS 65148-48-3), a certified reference material (molecular formula C5H4N4O, MW 136.11 g/mol) for analytical method development and quality control.
The molecular framework of [1,2,4]Triazolo[4,3-b]pyridazin-6-ol (C5H4N4O) is characterized by an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
| Molecular Formula | C5H4N4O |
| Molecular Weight | 136.11 g/mol |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | Light-sensitive; avoid direct strong light |
| Degree of Unsaturation (DBE) | 6.0 |
| UV Absorption | UV-absorbing |
| CAS Registry Year (est.) | 1975 |
| Nitrogen Atoms | 4 |