Methotrexate Impurity 61 CAS 65148-63-2
| CAS No. | 65148-63-2 |
| Molecular Formula | C22H26N8O5 |
| Molecular Weight | 482.49 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
CATO supplies Methotrexate Impurity 61 (CAS 65148-63-2), a pharmacopoeial impurity reference standard (molecular formula C22H26N8O5, MW 482.49 g/mol) used for the identification and quantification of trace impurities in APIs.
The molecular framework of Methotrexate Impurity 61 (C22H26N8O5) is characterized by a flavonoid core, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
This reference standard supports related-substances testing and method development in line with ICH Q3A/Q3B guidance on impurity control, and is suitable for system suitability testing and forced-degradation studies.
As a certified reference material (CRM), Methotrexate Impurity 61 meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
Physical & Chemical Properties
| Molecular Formula | C22H26N8O5 |
| Molecular Weight | 482.49 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 14.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1975 |
| Nitrogen Atoms | 8 |
Related Methotrexate Standards
- Methotrexate impurity N3 (CAS 65148-64-3)
- Methotrexate EP Impurity J (CAS 34378-65-9)
- Methotrexate impurity 50 (CAS CATO-C4X-115450)
- Methotrexate impurity 53 (CAS CATO-C4X-115453)
- Methotrexate Impurity 18 (CAS CATO-C4X-115418)
- Methotrexate Dimethylamide (CAS 71074-44-7)
- Methotrexate-d (CAS 432545-60-3)
- Methotrexate Impurity 28 (CAS 5939-37-7)
Recommended Standards
- Pemetrexed Impurity 54 (CAS 1265908-58-4)
- Fmoc-β-Ala-Gly-Gly-Gly-OH (CAS CATO-CPTD788410)
- Diundecylsulfane (CAS 35599-82-7)
- 2,2,2-Trichloro-N-{1-[(2,6-dichlorophenyl)methyl]-2-oxo-5-(trifluoromethyl)-1,2-dihydropyridin-3-yl}acetamide (CAS 339025-23-9)
- Fluoxetine Impurity 15 (CAS CATO-C4X-135215)
- Pyridoxine /Vitamin B6 Impurity 67 (CAS CATO-C4X-187767)
- Letrozole Impurity 29 (CAS 612-13-5)
- 2-(4-Chloro-2-methylphenoxy)-N-(furan-2-ylmethyl)acetamide (CAS 303118-75-4)
- 2'-Fluoro-2'-deoxy-ara-U-3'-phosphoramidite (CAS 1190089-70-3)
- Fmoc-β-Ala-D-2-Nal-OH (CAS CATO-CPTD788342)
- 5-cyclopropyl-3-(3-piperidyl)-1,2,4-oxadiazole (CAS 1339428-26-0)
- 3-((4-Amino-2-methylpyrimidin-5-yl)methyl)-5-(2-((hydroxy(phosphonooxy)phosphoryl)oxy)ethyl)-2-(1-hydroxyethyl)-4-methylthiazol-3-ium Chloride (CAS 20319-27-1)
- tert-Butyl 2-(2-iodophenoxy)acetate (CAS 1229805-95-1)
- Akt/SKG Substrate Peptide (CAS 276680-69-4)
Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
- Worldwide shipping with full export documentation (HS code guidance available)
- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com