3β-Hydroxy-5-cholestenoic Acid CAS 6561-58-6
| CAS No. | 6561-58-6 |
| Molecular Formula | C27H44O3 |
| Molecular Weight | 416.64 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
3β-Hydroxy-5-cholestenoic Acid (CAS 6561-58-6) is a high-purity analytical reference standard with the molecular formula C27H44O3 and a molecular weight of 416.64 g/mol.
Based on its molecular formula C27H44O3, 3β-Hydroxy-5-cholestenoic Acid contains a steroid core, a hydroxyl group, an ether linkage; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 416.64 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. The high carbon-to-hydrogen ratio imparts good lipophilicity, allowing liquid-liquid extraction with non-polar solvents such as n-hexane or ethyl acetate.
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
As a certified reference material (CRM), 3β-Hydroxy-5-cholestenoic Acid meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
Physical & Chemical Properties
| Molecular Formula | C27H44O3 |
| Molecular Weight | 416.64 g/mol |
| Appearance | Off-white to pale yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store at room temperature, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in chloroform, dichloromethane, ethyl acetate, DMSO |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light |
| Degree of Unsaturation (DBE) | 6.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1958 |
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Recommended Standards
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- 2-(3-oxomorpholin-4-yl)acetic acid (CAS 933692-47-8)
- 191036-43-8 4,6-Dideoxy-2,3-bis-O-(phenylmethyl)-L-xylo-hexose 4-Deoxy-2,3-di-O-benzyl-L-fucose (CAS 191036-43-8)
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