2-(4-Oxo-1-phenyl-1,4-dihydro-5h-pyrazolo[3,4-d]pyrimidin-5-yl)acetohydrazide CAS 656831-86-6

Description

Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including 2-(4-Oxo-1-phenyl-1,4-dihydro-5h-pyrazolo[3,4-d]pyrimidin-5-yl)acetohydrazide.

CATO supplies 2-(4-Oxo-1-phenyl-1,4-dihydro-5h-pyrazolo[3,4-d]pyrimidin-5-yl)acetohydrazide (CAS 656831-86-6), a certified reference material (molecular formula C13H12N6O2, MW 284.27 g/mol) for analytical method development and quality control.

Structurally, 2-(4-Oxo-1-phenyl-1,4-dihydro-5h-pyrazolo[3,4-d]pyrimidin-5-yl)acetohydrazide (C13H12N6O2) contains a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage. The molecule exhibits a degree of unsaturation (DBE) of 11.0, corresponding to approximately 11 ring(s) or double-bond equivalent(s). With a molecular weight of 284.27 g/mol, it falls into the medium-molecular-weight range. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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