Diethyl 2-(((3-(7-oxo-4,7-dihydro-1H-[1,2,3]triazolo[4,5-d]pyrimidin-5-yl)-4-propoxyphenyl)amino)methylene)malonate CAS 66975-54-0

Description

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

Diethyl 2-(((3-(7-oxo-4,7-dihydro-1H-[1,2,3]triazolo[4,5-d]pyrimidin-5-yl)-4-propoxyphenyl)amino)methylene)malonate (CAS 66975-54-0) is a high-purity analytical reference standard with the molecular formula C21H24N6O6 and a molecular weight of 456.45 g/mol.

The molecular framework of Diethyl 2-(((3-(7-oxo-4,7-dihydro-1H-[1,2,3]triazolo[4,5-d]pyrimidin-5-yl)-4-propoxyphenyl)amino)methylene)malonate (C21H24N6O6) is characterized by a flavonoid core, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

As a certified reference material (CRM), Diethyl 2-(((3-(7-oxo-4,7-dihydro-1H-[1,2,3]triazolo[4,5-d]pyrimidin-5-yl)-4-propoxyphenyl)amino)methylene)malonate meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

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