3-methyl-1H-pyrazolo[4,3-d]pyrimidine CAS 77816-07-0

Description

Compared with other compounds in its class, the molecular weight of 134.14 g/mol gives 3-methyl-1H-pyrazolo[4,3-d]pyrimidine distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

CATO supplies 3-methyl-1H-pyrazolo[4,3-d]pyrimidine (CAS 77816-07-0), a certified reference material (molecular formula C6H6N4, MW 134.14 g/mol) for analytical method development and quality control.

The molecular framework of 3-methyl-1H-pyrazolo[4,3-d]pyrimidine (C6H6N4) is characterized by a nitrile group, an aromatic ring system. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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