4-((2,4-Dihydroxybenzylidene)amino)-2-(3,4-dimethoxyphenethyl)isoindolin-1-one CAS 882747-50-4

Purity 97%+In StockPharmaceutical Standards
CAS No.882747-50-4
Molecular FormulaC25H24N2O5
Molecular Weight432.47 g/mol
CategoryPharmaceutical Standards / API Standards
AvailabilityIn Stock

Chemical Structure

4-((2,4-Dihydroxybenzylidene)amino)-2-(3,4-dimethoxyphenethyl)isoindolin-1-one CAS 882747-50-4 chemical structure

Description

CATO supplies 4-((2,4-Dihydroxybenzylidene)amino)-2-(3,4-dimethoxyphenethyl)isoindolin-1-one (CAS 882747-50-4), a certified reference material (molecular formula C25H24N2O5, MW 432.47 g/mol) for analytical method development and quality control.

The molecular framework of 4-((2,4-Dihydroxybenzylidene)amino)-2-(3,4-dimethoxyphenethyl)isoindolin-1-one (C25H24N2O5) is characterized by a flavonoid core, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

Molecular FormulaC25H24N2O5
Molecular Weight432.47 g/mol
AppearancePale yellow to yellow crystalline powder
Purity97%+
StorageStore in a cool place (≤25°C), protect from light, keep sealed and dry
Solubility (reference)Soluble in DMSO, DMF, methanol
StabilityMay oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening
Degree of Unsaturation (DBE)15.0
UV AbsorptionUV-absorbing
Structural FeaturesAromatic ring present
CAS Registry Year (est.)1987
Nitrogen Atoms2

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