tert-Butyl 2-(3-nitrobenzyl)piperazine-1-carboxylate CAS 886774-86-3

Description

Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including tert-Butyl 2-(3-nitrobenzyl)piperazine-1-carboxylate.

tert-Butyl 2-(3-nitrobenzyl)piperazine-1-carboxylate (CAS 886774-86-3), molecular formula C16H23N3O4 and molecular weight 321.37 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of tert-Butyl 2-(3-nitrobenzyl)piperazine-1-carboxylate (C16H23N3O4) is characterized by a flavonoid core, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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