3,5-Diphenyl-1H-pyrazolo[3,4-d]pyridazin-4(5H)-one CAS 90330-87-3

Description

Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including 3,5-Diphenyl-1H-pyrazolo[3,4-d]pyridazin-4(5H)-one.

3,5-Diphenyl-1H-pyrazolo[3,4-d]pyridazin-4(5H)-one (CAS 90330-87-3) is a high-purity analytical reference standard with the molecular formula C17H12N4O and a molecular weight of 288.30 g/mol.

The molecular framework of 3,5-Diphenyl-1H-pyrazolo[3,4-d]pyridazin-4(5H)-one (C17H12N4O) is characterized by a steroid core, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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