Methylene blue /Methylthioninium Chloride Impurity 25 CAS CATO-C4X-189625
| CAS No. | CATO-C4X-189625 |
| Molecular Formula | C14H14N3S |
| Molecular Weight | N/A |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including Methylene blue /Methylthioninium Chloride Impurity 25.
As a process- or degradation-related impurity, Methylene blue /Methylthioninium Chloride Impurity 25 (CAS CATO-C4X-189625, C14H14N3SHO4S) is essential for impurity profiling, related-substances testing, and forced-degradation studies in pharmaceutical development.
The molecular framework of Methylene blue /Methylthioninium Chloride Impurity 25 (C14H14N3SHO4S) is characterized by a nitrogen-containing heterocyclic scaffold, an amide group, a sulfone group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
This reference standard supports related-substances testing and method development in line with ICH Q3A/Q3B guidance on impurity control, and is suitable for system suitability testing and forced-degradation studies.
As a certified reference material (CRM), Methylene blue /Methylthioninium Chloride Impurity 25 meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
Physical & Chemical Properties
| Molecular Formula | C14H14N3S HO4S |
| Molecular Weight | 353.41 g/mol |
| Purity | 95% |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 9.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| Nitrogen Atoms | 3 |
| Sulfur Atoms | 2 |
Related Methylene Standards
- γ-Methylene-γ-butyrolactone (CAS 10008-73-8)
- 4,4＇-Methylene-Bis(2-chloroaniline) (CAS 101-14-4)
- 5-Methylene-2-phenyl-1,3-dioxolan-4-one (CAS 101822-11-1)
- 2-Methylene-1,3,6-trioxocane (CAS 105649-50-1)
- 6-Methyl-3-methylene-3,4-dihydroquinoxalin-2(1H)-one (CAS 106480-88-0)
- 5-Methylene-2(5H)-furanone (CAS 108-28-1)
- 3-(2-Methylene-4-phenylbutanoyl)oxazol-2(3H)-one (CAS 1151767-07-5)
- (Methylene-d2)gibberellin A5 (CAS 120396-32-9)
Recommended Standards
- Obeticholic Acid Impurity 39 (CAS 462122-38-9)
- Oxcarbazepine EP Impurity B (CAS 28721-09-7)
- Octreotide EP Impurity A Acetate (CAS CATO-C4X-15208)
- Isopropyl (tert-butoxycarbonyl)-L-serinate (CAS 1253690-12-8)
- 2-(Oxetan-2-yl)ethan-1-amine (CAS 1536914-60-9)
- 3-(Dimethyl(3-(4-octylbenzamido)propyl)ammonio)propane-1-sulfonate (CAS 216667-49-1)
- 3-Chloro-5-phenylisothiazole-4-carboxylic acid (CAS 86125-06-6)
- (S)-Methyl 2-ethylpyrrolidine-2-carboxylate (CAS 869058-61-7)
- 1-(5-Chloro-2-methylphenyl)-5-phenyl-4,5-dihydro-1h-pyrazole-3-carboxylic acid (CAS 1264047-45-1)
- Semaglutide impound 212 (CAS CATO-C4X-2226212)
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