Methylene blue Impurity 4 CAS CATO-C4X-18964
| CAS No. | CATO-C4X-18964 |
| Molecular Formula | C12H7INS |
| Molecular Weight | N/A |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
As a process- or degradation-related impurity, Methylene blue Impurity 4 (CAS CATO-C4X-18964, C12H7INSI) is essential for impurity profiling, related-substances testing, and forced-degradation studies in pharmaceutical development.
The molecular framework of Methylene blue Impurity 4 (C12H7INSI) is characterized by a nitrogen-containing heterocyclic scaffold, a nitrile group, sulfur-containing functionality, iodine substitution. In reversed-phase chromatography, its retention is governed primarily by its high hydrophobicity (logP), which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Halogen substitution (F/Cl/Br/I) produces a distinctive isotope pattern in mass spectrometry, a valuable aid for qualitative confirmation.
This reference standard supports related-substances testing and method development in line with ICH Q3A/Q3B guidance on impurity control, and is suitable for system suitability testing and forced-degradation studies.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C12H7INS I |
| Molecular Weight | 451.06 g/mol |
| Purity | >95% |
| Storage | Store at room temperature, protect from light |
| Solubility (reference) | Soluble in chloroform, dichloromethane, ethyl acetate |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light |
| Degree of Unsaturation (DBE) | 9.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| Halogen Content | I-containing |
| Nitrogen Atoms | 1 |
| Sulfur Atoms | 1 |
Related Methylene Standards
- γ-Methylene-γ-butyrolactone (CAS 10008-73-8)
- 4,4＇-Methylene-Bis(2-chloroaniline) (CAS 101-14-4)
- 5-Methylene-2-phenyl-1,3-dioxolan-4-one (CAS 101822-11-1)
- 2-Methylene-1,3,6-trioxocane (CAS 105649-50-1)
- 6-Methyl-3-methylene-3,4-dihydroquinoxalin-2(1H)-one (CAS 106480-88-0)
- 5-Methylene-2(5H)-furanone (CAS 108-28-1)
- 3-(2-Methylene-4-phenylbutanoyl)oxazol-2(3H)-one (CAS 1151767-07-5)
- (Methylene-d2)gibberellin A5 (CAS 120396-32-9)
Recommended Standards
- Salmeterol EP Impurity C (CAS 94749-11-8)
- N-nitroso Peramivir Impurity 97 (CAS CATO-C4X-183397)
- Methotrexate Impurity 35 (CAS CATO-C4X-115435)
- 3-(4-(5-Methylpyrimidin-4-yl)piperazine-1-carbonyl)-2H-chromen-2-one (CAS 1448054-88-3)
- (1-Bromo-6,7-dihydro-4H-thieno[3,4-c]pyran-4-yl)methanamine (CAS 1310427-41-8)
- 3-(3-Chloro-2-hydroxypropyl)thiazolidine-2,4-dione (CAS 141037-39-0)
- 2-(1-Butyl-2-phenyl-1H-benzo[d]imidazol-5-yl)acetic acid (CAS 62467-88-3)
- 3-[(1,3-dioxaindan-5-yloxy)methyl]-4-methoxybenzaldehyde (CAS 832739-19-2)
- N-[1-(3-Chloropropyl)piperidin-4-yl]-4-fluorobenzamide (CAS 72084-87-8)
- Tau Peptide (306-317) trifluoroacetate salt (CAS 1003007-59-7)
Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
- Worldwide shipping with full export documentation (HS code guidance available)
- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com