Enrofloxacin Impurity 12 CAS 130579-27-0
| CAS No. | 130579-27-0 |
| Molecular Formula | C13H10FNO4 |
| Molecular Weight | 263.22 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.
Enrofloxacin Impurity 12 (CAS 130579-27-0) is a certified pharmaceutical impurity reference standard with the molecular formula C13H10FNO4 and a molecular weight of 263.22 g/mol.
Enrofloxacin Impurity 12 (C13H10FNO4) contains a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage, fluorine substitution. It is notably hygroscopic, halogen substitution raises its density and boiling point, the carboxyl group imparts weak acidity; these physicochemical properties determine the optimal preparation and storage of its standard solutions. Halogen substitution (F/Cl/Br/I) produces a distinctive isotope pattern in mass spectrometry, a valuable aid for qualitative confirmation.
As a certified reference material (CRM), Enrofloxacin Impurity 12 meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
Physical & Chemical Properties
| Molecular Formula | C13H10FNO4 |
| Molecular Weight | 263.22 g/mol |
| Appearance | Pale yellow to yellow crystalline powder. (slight halide-like odor) |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry, keep tightly closed to prevent evaporation |
| Solubility (reference) | Soluble in methanol, acetonitrile, DMSO, water (slightly soluble) |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening; Contains ester bond; hydrolyzes under strong alkaline conditions |
| Degree of Unsaturation (DBE) | 9.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1994 |
| Halogen Content | F-containing |
| Nitrogen Atoms | 1 |
Related Enrofloxacin Standards
- Enrofloxacin Impurity 8 (CAS CATO-C4X-16288)
- Enrofloxacin Impurity 11 (CAS 160312-26-5)
- Enrofloxacin Impurity 13 (CAS 2388490-12-6)
- Enrofloxacin Impurity 7 (CAS 93107-31-4)
- Enrofloxacin EP Impurity F (CAS 131775-99-0)
- Enrofloxacin EP Impurity C (CAS 138892-82-7)
- Enrofloxacin - Impurity C (Hydrochloride Salt) (CAS 2732310-93-7)
- Enrofloxacin Impurity 10 (CAS CATO-C4X-162810)
Recommended Standards
- Levofloxacin impurity 71 (CAS CATO-C4X-137171)
- Nemonoxacin Impurity 27 (CAS 926279-65-4)
- Levofloxacin Impurity 43 (CAS 117620-84-5)
- Nemonoxacin Impurity 41 (CAS CATO-C4X-312841)
- SAR107375E Impurity 8 (CAS 42454-54-6)
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- Etoricoxib Impurity 85 (CAS CATO-C4X-108085)
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