Fluorescein Impurity 3 CAS 1428447-91-9

Description

Compared with other compounds in its class, the molecular weight of 350.32 g/mol gives Fluorescein Impurity 3 distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

Fluorescein Impurity 3 (CAS 1428447-91-9) is a certified pharmaceutical impurity reference standard with the molecular formula C20H14O6 and a molecular weight of 350.32 g/mol.

Owing to a flavonoid core, a hydroxyl group, an ether linkage, Fluorescein Impurity 3 is typically pale yellow to yellow crystalline powder. Its solid-state physicochemical attributes — including a molecular weight of 350.32 g/mol — guide the selection of appropriate sample preparation and analytical techniques. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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