Metronidazole Impurity 25 CAS 16156-93-7
| CAS No. | 16156-93-7 |
| Molecular Formula | C8H12ClN3O3 |
| Molecular Weight | 233.65 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
Structurally, Metronidazole Impurity 25 (C8H12ClN3O3) contains a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage, chlorine substitution. The molecule exhibits a degree of unsaturation (DBE) of 4.0, corresponding to approximately 4 ring(s) or double-bond equivalent(s). With a molecular weight of 233.65 g/mol, it falls into the medium-molecular-weight range. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
As a process- or degradation-related impurity, Metronidazole Impurity 25 (CAS 16156-93-7, C8H12ClN3O3) is essential for impurity profiling, related-substances testing, and forced-degradation studies in pharmaceutical development.
This reference standard supports related-substances testing and method development in line with ICH Q3A/Q3B guidance on impurity control, and is suitable for system suitability testing and forced-degradation studies.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C8H12ClN3O3 |
| Molecular Weight | 233.65 g/mol |
| Appearance | Off-white to pale yellow crystalline powder. (slight halide-like odor, faint amine-like odor) |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry, keep tightly closed to prevent evaporation |
| Solubility (reference) | Soluble in methanol, ethanol, DMSO |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening; Contains ester bond; hydrolyzes under strong alkaline conditions |
| Degree of Unsaturation (DBE) | 4.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1961 |
| Halogen Content | Cl-containing |
| Nitrogen Atoms | 3 |
Related Metronidazole Standards
- Metronidazole Impurity 20 (CAS 19765-12-9)
- Metronidazole EP Impurity F (CAS 16156-94-8)
- Metronidazole Acetate (CAS 13182-82-6)
- Metronidazole Impurity 12 (CAS CATO-C4X-140512)
- Metronidazole Impurity 9 (CAS 87009-72-1)
- Metronidazole benzoate (CAS 13182-89-3)
- Metronidazole-hydroxy D2 (CAS 2196180-19-3)
- Metronidazole Impurity 10 (CAS 56750-04-0)
Recommended Standards
- 8-Chloro-3-methyl-2,5-dihydro-4H-pyrazolo[4,3-c]quinolin-4-one (CAS 848818-35-9)
- 5-Chloro-9H-pyrimido[4,5-b]indole-2,4-diamine (CAS 1126602-48-9)
- 7-chloro-8-methoxy-1H-pyrido[4,3-d]pyrimidine-2,4-dione (CAS 2454491-07-5)
- 4-Chloro-6-(1-methyl-1H-pyrazol-4-yl)pyrrolo[2,1-f][1,2,4]triazine (CAS 1703794-33-5)
- Methotrexate Impurity 45 (CAS CATO-C4X-115445)
- Cefotiam Impurity 38 (CAS CATO-C4X-120438)
- Bilastine Impurity 47 (CAS CATO-C4X-225347)
- 1-(2,4-Dimethylphenyl)-3-(p-tolyl)propan-1-one (CAS 898768-79-1)
- Nicorandil Impurity 70 (CAS CATO-C4X-190770)
- 4,6-Dichloro-2,5-diphenylpyrimidine (CAS 29133-99-1)
- 3-(2-Methoxyethyl)-2-methyl-5-phenylbenzo[d]oxazol-3-ium 4-methylbenzenesulfonate (CAS 63979-86-2)
- (2S,5R,6R)-6-((R)-2-((Furan-2-carbonyl)oxy)-4-methylpentanamido)-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid (CAS 54340-65-7)
- Methyl 2-amino-2-(4-bromophenyl)acetate hydrochloride (CAS 42718-20-7)
- CGMP Dependent Kinase Inhibitor Peptide (CAS 82801-73-8)
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