Cefazolin Impurity 53 CAS 26476-16-4

Purity >95%In StockPharmacopoeial Impurity Standards
CAS No.26476-16-4
Molecular FormulaC3H5N5O
Molecular Weight127.10 g/mol
CategoryPharmacopoeial Impurity Standards / EP Impurities
AvailabilityIn Stock

Chemical Structure

Cefazolin Impurity 53 CAS 26476-16-4 chemical structure

Description

The occurrence of Cefazolin Impurity 53 is closely related to an amide group, a hydroxyl group, an ether linkage in its molecular formula, which determines its behaviour during synthesis, purification, or degradation.

Cefazolin Impurity 53 (CAS 26476-16-4) is a certified pharmaceutical impurity reference standard with the molecular formula C3H5N5O and a molecular weight of 127.10 g/mol.

The molecular framework of Cefazolin Impurity 53 (C3H5N5O) is characterized by an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.

Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.

CATO manufactures Cefazolin Impurity 53 under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.

Physical & Chemical Properties

Molecular FormulaC3H5N5O
Molecular Weight127.10 g/mol
Purity>95%
StorageStore at 2-8°C, protect from light
Solubility (reference)Soluble in DMSO, DMF, methanol
StabilityLight-sensitive; avoid direct strong light
Degree of Unsaturation (DBE)4.0
UV AbsorptionUV-absorbing
CAS Registry Year (est.)1964
Nitrogen Atoms5

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