Cholic Acid impurity 75 CAS 2862-62-6

Purity 97%+In StockPharmacopoeial Impurity Standards
CAS No.2862-62-6
Molecular FormulaC25H40O4
Molecular Weight404.58 g/mol
CategoryPharmacopoeial Impurity Standards / EP Impurities
AvailabilityIn Stock

Chemical Structure

Cholic Acid impurity 75 CAS 2862-62-6 chemical structure

Description

Compared with other compounds in its class, the molecular weight of 404.58 g/mol gives Cholic Acid impurity 75 distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

CATO supplies Cholic Acid impurity 75 (CAS 2862-62-6), a pharmacopoeial impurity reference standard (molecular formula C25H40O4, MW 404.58 g/mol) used for the identification and quantification of trace impurities in APIs.

Owing to a steroid core, a hydroxyl group, an ether linkage, Cholic Acid impurity 75 is typically off-white to pale yellow crystalline powder. Its solid-state physicochemical attributes — including a molecular weight of 404.58 g/mol — guide the selection of appropriate sample preparation and analytical techniques. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

Molecular FormulaC25H40O4
Molecular Weight404.58 g/mol
AppearanceOff-white to pale yellow crystalline powder
Purity97%+
StorageStore at room temperature, protect from light, keep sealed and dry
Solubility (reference)Soluble in chloroform, dichloromethane, ethyl acetate, DMSO
StabilityMay oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light
Degree of Unsaturation (DBE)6.0
UV AbsorptionUV-absorbing
Structural FeaturesAromatic ring present
CAS Registry Year (est.)1957

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