Temozolomide Impurity 2 CAS 287964-59-4
| CAS No. | 287964-59-4 |
| Molecular Formula | C6H4N6O4 |
| Molecular Weight | 224.13 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
As a process- or degradation-related impurity, Temozolomide Impurity 2 (CAS 287964-59-4, C6H4N6O4) is essential for impurity profiling, related-substances testing, and forced-degradation studies in pharmaceutical development.
The molecular framework of Temozolomide Impurity 2 (C6H4N6O4) is characterized by an amino-acid-derived structure, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
This reference standard supports related-substances testing and method development in line with ICH Q3A/Q3B guidance on impurity control, and is suitable for system suitability testing and forced-degradation studies.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C6H4N6O4 |
| Molecular Weight | 224.13 g/mol |
| Appearance | Pale yellow to yellow crystalline powder. (faint amine-like odor) |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in methanol, ethanol, DMSO, water |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 8.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 2025 |
| Nitrogen Atoms | 6 |
Related Temozolomide Standards
- Temozolomide Impurity 26 (CAS 13114-72-2)
- Temozolomide Impurity 25 (CAS 83-01-2)
- Temozolomide Impurity 15 (CAS 100607-08-7)
- Temozolomide-d3 (CAS 208107-14-6)
- Temozolomide EP Impurity B (CAS 113942-30-6)
- Temozolomide Impurity 19 (CAS CATO-C4X-135319)
- Temozolomide Impurity 3 (CAS 188612-53-5)
- Temozolomide EP Impurity C (CAS 114601-31-9)
Recommended Standards
- Pentafluoroethyl phenyl ketone (CAS 394-52-5)
- 2-(4-Nitro-3-(trifluoromethyl)-1H-pyrazol-1-yl)ethan-1-amine (CAS 1006440-52-3)
- Allyl pentafluorophenyl ether (CAS 33104-02-8)
- Ethyl 2-cyano-2-(2,2,2-trifluoroacetamido)acetate (CAS 1008711-58-7)
- Trilostane Impurity 3 (CAS CATO-C4X-21373)
- Pemafibrate Impurity 102 (CAS CATO-C4X-2903102)
- Salmeterol EP Impurity D (CAS 1391052-04-2)
- 2-Oxo-1-phenyl-1,2,4,5,6,7-hexahydropyrazolo[1,5-a]pyridine-3-carboxylic acid (CAS 1818871-26-9)
- (2-(Phenylthio)pyridin-3-yl)methanol (CAS 43003-89-0)
- Tryptophan Impurity 9 (CAS 141215-69-2)
- 2-(Diisobutylamino)-2-phenylacetic acid (CAS 1103584-13-9)
- 1-(3-Cyclopropyl-3-hydroxypropyl)-3-(thiophen-2-yl)urea (CAS 1396680-00-4)
- 4-(2-Bromophenyl)butan-2-one (CAS 3506-68-1)
- Hexapeptide-9 (CAS 1228371-11-6)
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