Metformin EP Impurity B Nitrate CAS 3553-49-9
| CAS No. | 3553-49-9 |
| Molecular Formula | C4H8N8 |
| Molecular Weight | N/A |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.
Metformin EP Impurity B Nitrate (CAS 3553-49-9) is a certified pharmaceutical impurity reference standard with the molecular formula C4H8N8HNO3 and a molecular weight of 231.18 g/mol.
Metformin EP Impurity B Nitrate (C4H8N8HNO3) contains an amino-acid-derived structure, an amide group, a hydroxyl group, an ether linkage. It is notably hygroscopic, the carboxyl group imparts weak acidity; these physicochemical properties determine the optimal preparation and storage of its standard solutions. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
As a certified reference material (CRM), Metformin EP Impurity B Nitrate meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
Physical & Chemical Properties
| Molecular Formula | C4H8N8 HNO3 |
| Molecular Weight | 231.18 g/mol |
| Appearance | Off-white to pale yellow crystalline powder. (faint amine-like odor) |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in methanol, ethanol, DMSO, water |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 5.0 |
| UV Absorption | UV-absorbing |
| CAS Registry Year (est.) | 1958 |
| Nitrogen Atoms | 9 |
Related Metformin Standards
- Metformin EP Impurity D (CAS 108-78-1)
- Metformin Hydrochloride (CAS 1115-70-4)
- Metformin-d6 Hydrochloride (CAS 1185166-01-1)
- Metformin Impurity 38 (CAS 118632-64-7)
- Metformin EP impurity F Tetrahydrofuran Solution (CAS 124-40-3 (free base))
- Metformin Impurity 532 (CAS 141-83-3)
- Metformin Impurity N74 (CAS 1609-00-3)
- Metformin EP Impurity E HCl (CAS 1674-62-0)
Recommended Standards
- Flubendazole EP Impurity D (CAS 1515807-30-3)
- Atrasentan Impurity 37 (CAS 1058393-04-6)
- Ceftiofur Impurity 11 (CAS CATO-C4X-158111)
- Methyl 4-fluoro-3-(4-methylphenyl)benzoate (CAS 1381944-67-7)
- 2,5-Dichloro-4-nitro-benzoyl Chloride (CAS 148428-80-2)
- 5-Iodo-1-methyl-1H-pyrazolo[3,4-b]pyridin-3-amine (CAS 766515-35-9)
- 1-Boc-N-(4-pyridylmethyl)piperidin-4-amine (CAS 206274-24-0)
- 1-(1-(Aminomethyl)-3-ethylcyclopentyl)ethan-1-ol (CAS 1559018-39-1)
- 5-(Chloromethyl)-n-(4-ethylphenyl)-1,3,4-thiadiazole-2-carboxamide (CAS 1087792-49-1)
- Semaglutide impurity 25 (CAS CATO-C4X-222625)
Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
- Worldwide shipping with full export documentation (HS code guidance available)
- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com