| CAS No. | 477850-93-4 |
| Molecular Formula | C16H13N3O2 |
| Molecular Weight | 279.29 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including 4-{[(4-phenoxyphenyl)amino]methylidene}-4,5-dihydro-1H-pyrazol-5-one.
CATO supplies 4-{[(4-phenoxyphenyl)amino]methylidene}-4,5-dihydro-1H-pyrazol-5-one (CAS 477850-93-4), a certified reference material (molecular formula C16H13N3O2, MW 279.29 g/mol) for analytical method development and quality control.
4-{[(4-phenoxyphenyl)amino]methylidene}-4,5-dihydro-1H-pyrazol-5-one (C16H13N3O2) contains a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage. It is notably hygroscopic, the carboxyl group imparts weak acidity; these physicochemical properties determine the optimal preparation and storage of its standard solutions. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
As a certified reference material (CRM), 4-{[(4-phenoxyphenyl)amino]methylidene}-4,5-dihydro-1H-pyrazol-5-one meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
| Molecular Formula | C16H13N3O2 |
| Molecular Weight | 279.29 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 12.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 2025 |
| Nitrogen Atoms | 3 |