4-Hydroxy-1H-pyrazol-3(2H)-one CAS 53666-86-7
| CAS No. | 53666-86-7 |
| Molecular Formula | C3H4N2O2 |
| Molecular Weight | 100.08 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
Recommended for analytical method development and validation; each product is supplied with a full Certificate of Analysis (COA) ensuring ISO 17034 metrological traceability.
4-Hydroxy-1H-pyrazol-3(2H)-one (CAS 53666-86-7), molecular formula C3H4N2O2 and molecular weight 100.08 g/mol, is a CATO reference standard with documented purity and traceability.
The molecular framework of 4-Hydroxy-1H-pyrazol-3(2H)-one (C3H4N2O2) is characterized by an amino-acid-derived structure, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C3H4N2O2 |
| Molecular Weight | 100.08 g/mol |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 3.0 |
| UV Absorption | UV-absorbing |
| CAS Registry Year (est.) | 1972 |
| Nitrogen Atoms | 2 |
Related Hydroxy Standards
- 1-Hydroxy-1,3-dihydro-2H-imidazol-2-one (CAS 116451-44-6)
- 1-Hydroxy-1H-imidazol-3-ium-3-olate (CAS 35321-46-1)
- 3-Hydroxy-4-methyl-1,2,5-oxadiazole (CAS 292856-44-1)
- 5-Hydroxy-3-methyl-1,2,4-oxadiazole (CAS 52386-40-0)
- 4-Hydroxy-2-butynoic Acid (CAS 7218-52-2)
- 1345994-72-0 4-hydroxy-3-cyclobutene-1,2-dione-1,4 13c2 (CAS 1345994-72-0)
- N’-hydroxy-2-methylprop-2-enimidamide (CAS 1369871-98-6)
- 3-hydroxy-3-methyl-cyclobutanone (CAS 179073-80-4)
Recommended Standards
- 5-Aminoisoxazol-3(2H)-one (CAS 822-63-9)
- 2-Aminooxazol-5(4H)-one (CAS 94134-26-6)
- 2-Amino-2-cyanoacetic acid (CAS 6232-21-9)
- 5-Methyl-1,3,4-oxadiazol-2(3H)-one (CAS 3069-67-8)
- 2-Oxepanone, polymer with 2,2-dimethyl-1,3-propanediol and 1,1'-methylenebis[isocyanatobenzene] (CAS 153965-61-8)
- 1-(2-furanylmethyl)piperazine dihydrochloride 1-(furan-2-ylmethyl)piperazine dihydrochloride 59037-70-6 A832125 (CAS 59037-70-6)
- di-tert-Butyl 6-oxo-1,4-diazepane-1,4-dicarboxylate (CAS 459417-40-4)
- 1-Methyl-9H-pyrido[3,4-b]indole-3-carboxylic acid (CAS 22329-38-0)
- 5-Chloro-3-isopropyl-1H-indole (CAS 1441421-47-1)
- 1-(5-Chloro-2-ethoxyphenyl)-N-methylmethanamine (CAS 893581-50-5)
- 2-(tert-Butyl)-6-isopropyl-4-(pyridazin-3-ylamino)phenol (CAS 114548-84-4)
- (4-methoxyphenyl)[4-(trifluoromethyl)phenyl]methanamine hydrochloride (CAS 1443980-52-6)
- 4-Methylthiophen-3-ol (CAS 1261433-53-7)
- Peptide Fmoc-Thi-Thi-OH (CAS CATO-CPTD788552)
Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
- Worldwide shipping with full export documentation (HS code guidance available)
- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com