4,4'-Bis(4-amino-1-naphthylazo)-2,2'-stilbenedisulfonic Acid CAS 5463-64-9

Purity Technical GradeIn StockPharmaceutical Standards
CAS No.5463-64-9
Molecular FormulaC34H26N6O6S2
Molecular Weight678.74 g/mol
CategoryPharmaceutical Standards / API Standards
AvailabilityIn Stock

Chemical Structure

4,4'-Bis(4-amino-1-naphthylazo)-2,2'-stilbenedisulfonic Acid CAS 5463-64-9 chemical structure

Description

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

4,4'-Bis(4-amino-1-naphthylazo)-2,2'-stilbenedisulfonic Acid (CAS 5463-64-9), molecular formula C34H26N6O6S2 and molecular weight 678.74 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of 4,4'-Bis(4-amino-1-naphthylazo)-2,2'-stilbenedisulfonic Acid (C34H26N6O6S2) is characterized by a nitrogen-containing heterocyclic scaffold, an amide group, a sulfone group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

As a certified reference material (CRM), 4,4'-Bis(4-amino-1-naphthylazo)-2,2'-stilbenedisulfonic Acid meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

Molecular FormulaC34H26N6O6S2
Molecular Weight678.74 g/mol
PurityTechnical Grade
StorageStore in a cool place (≤25°C), protect from light, keep sealed and dry
Solubility (reference)Soluble in DMSO, DMF, methanol
StabilityMay oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening
Degree of Unsaturation (DBE)25.0
UV AbsorptionUV-absorbing
Structural FeaturesAromatic ring present
CAS Registry Year (est.)1958
Nitrogen Atoms6
Sulfur Atoms2

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