Methotrexate Impurity 44 CAS 93528-16-6
| CAS No. | 93528-16-6 |
| Molecular Formula | C13H14N2O6 |
| Molecular Weight | 294.26 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
Owing to a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage, Methotrexate Impurity 44 is typically pale yellow to yellow crystalline powder. Its solid-state physicochemical attributes — including a molecular weight of 294.26 g/mol — guide the selection of appropriate sample preparation and analytical techniques. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
As a process- or degradation-related impurity, Methotrexate Impurity 44 (CAS 93528-16-6, C13H14N2O6) is essential for impurity profiling, related-substances testing, and forced-degradation studies in pharmaceutical development.
This reference standard supports related-substances testing and method development in line with ICH Q3A/Q3B guidance on impurity control, and is suitable for system suitability testing and forced-degradation studies.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C13H14N2O6 |
| Molecular Weight | 294.26 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 8.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1983 |
| Nitrogen Atoms | 2 |
Related Methotrexate Standards
- Methotrexate Impurity 42 (CAS 79974-19-9)
- Methotrexate Impurity 43 (CAS 79974-20-2)
- Methotrexate Impurity 66 (CAS CATO-C4X-115466)
- Methotrexate Impurity 68 (CAS CATO-C4X-115468)
- Methotrexate Impurity 70 (CAS CATO-C4X-115470)
- Methotrexate Impurity 77 (CAS CATO-C4X-115477)
- Methotrexate Impurity 34 (CAS CATO-C4X-115434)
- Methotrexate Impurity 40 (CAS 94807-78-0)
Recommended Standards
- rac-Tenofovir-d7 (CAS CATO-C4X-107643)
- Dolutegravir Impurity 67 (CAS CATO-C4X-162067)
- Tenofovir Impurity 37 (CAS 161760-03-8)
- Pimobendan Impurity 8 (CAS CATO-C4X-24618)
- Ampicillin Impurity 76 (CAS CATO-C4X-144376)
- Doxylamine EP Impurity C HCI (CAS 1221-70-1（free base）)
- Dutaverine Impurity 33 HCl (CAS CATO-C4X-243533)
- 2-(2-(2,3,4-Trifluorophenoxy)ethyl)-1,3-dioxane (CAS 1443341-68-1)
- 4'-Chloro-3-hydroxy-5-(trifluoromethyl)-[1,1'-biphenyl]-4-carbonitrile (CAS 914637-19-7)
- 2-(4-(tert-Butyl)benzyl)-2H-benzo[e][1,2,3,4]thiatriazine 1,1-dioxide (CAS 866151-97-5)
- Nitrendipine impurity 20 (CAS CATO-C4X-183420)
- tert-Butyl 4-((2-chloro-6-methylpyrimidin-4-yl)oxy)piperidine-1-carboxylate, 95% (CAS 1289386-94-2)
- N-(2-aminoethyl)pyridine-3-sulfonamide (CAS 1016797-15-1)
- Semaglutide impurity 24 (CAS CATO-C4X-222624)
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