Methotrexate Impurity 82 CAS CATO-C4X-115482
| CAS No. | CATO-C4X-115482 |
| Molecular Formula | C29H31N9O5 |
| Molecular Weight | 585.61 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
As a process- or degradation-related impurity, Methotrexate Impurity 82 (CAS CATO-C4X-115482, C29H31N9O5) is essential for impurity profiling, related-substances testing, and forced-degradation studies in pharmaceutical development.
Methotrexate Impurity 82 (C29H31N9O5) contains a steroid core, an amide group, a hydroxyl group, an ether linkage. It is notably hygroscopic, the carboxyl group imparts weak acidity; these physicochemical properties determine the optimal preparation and storage of its standard solutions. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
This reference standard supports related-substances testing and method development in line with ICH Q3A/Q3B guidance on impurity control, and is suitable for system suitability testing and forced-degradation studies.
As a certified reference material (CRM), Methotrexate Impurity 82 meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
Physical & Chemical Properties
| Molecular Formula | C29H31N9O5 |
| Molecular Weight | 585.61 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 19.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| Nitrogen Atoms | 9 |
Related Methotrexate Standards
- Methotrexate EP Impurity G (CAS 2519848-42-9)
- Methotrexate Diglutamate (CAS 41600-13-9)
- Methotrexate Impurity 72 (CAS CATO-C4X-115472)
- Methotrexate Impurity 73 (CAS CATO-C4X-115473)
- Methotrexate impurity 57 (CAS CATO-C4X-115457)
- Methotrexate Impurity 83 (CAS CATO-C4X-115483)
- Methotrexate impurity 52 (CAS CATO-C4X-115452)
- Methotrexate Diethyl Ester (CAS 43170-88-3)
Recommended Standards
- Fosamprenavir (CAS 226700-79-4)
- Nafamostat Impurity 27 (CAS CATO-C4X-133227)
- Amikacin (CAS 37517-28-5)
- Nafamostat impurity 87 (CAS CATO-C4X-133287)
- Droperidol Impurity 19 (CAS CATO-C4X-276919)
- Esmolol Impurity 23 (CAS CATO-C4X-189123)
- Ixazomib Impurity 60 (CAS CATO-C4X-126760)
- 1-(3-Chloro-5-(trifluoromethyl)pyridin-2-yl)-4,4,4-trifluorobutane-1,3-dione (CAS 207994-03-4)
- 2-(Difluoromethyl)-4,5-difluoropyridin-3-ol (CAS 1804453-95-9)
- 4-Bromo-5-chloro-2-methylbenzenethiol (CAS 1208076-88-3)
- N-(4-ethoxyphenyl)indoline-1-carboxamide (CAS 904808-01-1)
- Norepinephrine Impurity 42 (CAS 28822-73-3)
- Methyl 2-bromo-3,3-dimethoxypropanoate (CAS 191330-98-0)
- a-Bag Cell Peptide (1-8) (CAS 87549-53-9)
Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
- Worldwide shipping with full export documentation (HS code guidance available)
- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com