Methotrexate Diglutamate CAS 41600-13-9
| CAS No. | 41600-13-9 |
| Molecular Formula | C25H29N9O8 |
| Molecular Weight | 583.55 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
Methotrexate Diglutamate (CAS 41600-13-9), molecular formula C25H29N9O8 and molecular weight 583.55 g/mol, is a CATO reference standard with documented purity and traceability.
Owing to a flavonoid core, an amide group, a hydroxyl group, an ether linkage, Methotrexate Diglutamate is typically pale yellow to yellow crystalline powder. Its solid-state physicochemical attributes — including a molecular weight of 583.55 g/mol — guide the selection of appropriate sample preparation and analytical techniques. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C25H29N9O8 |
| Molecular Weight | 583.55 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 16.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1968 |
| Nitrogen Atoms | 9 |
Related Methotrexate Standards
- Methotrexate Impurity 82 (CAS CATO-C4X-115482)
- Methotrexate EP Impurity G (CAS 2519848-42-9)
- Methotrexate Impurity 72 (CAS CATO-C4X-115472)
- Methotrexate Impurity 73 (CAS CATO-C4X-115473)
- Methotrexate impurity 57 (CAS CATO-C4X-115457)
- Methotrexate Impurity 83 (CAS CATO-C4X-115483)
- Methotrexate impurity 52 (CAS CATO-C4X-115452)
- Methotrexate Diethyl Ester (CAS 43170-88-3)
Recommended Standards
- Lenalidomide Lactosamine Adduct (Mixture of Diastereomers) (CAS CATO-C4X-190373)
- Metoclopramide Impurity 123 (CAS CATO-C4X-1415123)
- 3'-De(phosphinicooxy)guanylyl-1H-1,2,3-triazole-1,4-diyl-(3''→5')-2',5'-dideoxy-guanosine (CAS 1513874-09-3)
- Metoclopramide impurity 52 (CAS CATO-C4X-141552)
- 2-(5-Tetrazolyl)ethanamine (CAS 31602-64-9)
- 8-Mercapto-1,3,7-trimethyl-3,7-dihydro-1H-purine-2,6-dione (CAS 1790-74-5)
- 2-Chloro-4-(chloromethoxy)-1-nitrobenzene (CAS 1537788-40-1)
- n-{2-[(5-bromopyridin-2-yl)amino]ethyl}acetamide (CAS 1157002-06-6)
- 3-(1,2-Dimethyl-3-propylpyrrolidin-3-yl)phenol (CAS 1505-26-6)
- 1-[2-(propan-2-yloxy)ethyl]piperidin-4-ol (CAS 1247662-19-6)
- Benzo[d]isothiazole-7-carbaldehyde (CAS 1354746-44-3)
- 1-(3-Methoxypropyl)pyrrolidin-2-one (CAS 157769-80-7)
- Ethyl 7-methyl-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)imidazo[1,2-a]pyridine-2-carboxylate (CAS 2377610-47-2)
- Peptide Impurity 81 TFA (CAS CATO-CPTD788581)
Why CATO?
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