Methotrexate impurity 52 CAS CATO-C4X-115452
| CAS No. | CATO-C4X-115452 |
| Molecular Formula | C20H20N10O7 |
| Molecular Weight | 512.44 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Methotrexate impurity 52 (CAS CATO-C4X-115452) is a certified pharmaceutical impurity reference standard with the molecular formula C20H20N10O7 and a molecular weight of 512.44 g/mol.
The molecular framework of Methotrexate impurity 52 (C20H20N10O7) is characterized by a flavonoid core, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).
This reference standard supports related-substances testing and method development in line with ICH Q3A/Q3B guidance on impurity control, and is suitable for system suitability testing and forced-degradation studies.
Physical & Chemical Properties
| Molecular Formula | C20H20N10O7 |
| Molecular Weight | 512.44 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 16.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| Nitrogen Atoms | 10 |
Related Methotrexate Standards
- Methotrexate Diethyl Ester (CAS 43170-88-3)
- Methotrexate Diethyl Ester (CAS CATO-CIM-105887)
- Methotrexate Dimethylamide Sodium Salt (CAS CATO-C4X-115436)
- Methotrexate impurity 51 (CAS CATO-C4X-115451)
- Methotrexate Impurity 83 (CAS CATO-C4X-115483)
- Methotrexate-d (CAS 432545-60-3)
- Methotrexate impurity 57 (CAS CATO-C4X-115457)
- Methotrexate impurity 50 (CAS CATO-C4X-115450)
Recommended Standards
- 1,1,2,2-Tetrakis(4-nitrophenyl)ethene (CAS 47797-98-8)
- 5,5'-(Benzo[c][1,2,5]thiadiazole-4,7-diylbis(ethyne-2,1-diyl))diisophthalicacid (CAS 2049078-65-9)
- 6-(2,6-Dichlorophenyl)-2-((4-(2-(diethylamino)ethoxy)phenyl)amino)-8-methylpyrido[2,3-d]pyrimidin-7(8H)-one (CAS 185039-89-8)
- Gentiournoside D (CAS 157722-21-9)
- Deucravacitinib Impurity 4 (CAS CATO-C4X-31384)
- Dotinurad Impurity 77 (CAS 35458-33-4)
- Chlorphenamine Impurity 22 (CAS 4584-46-7)
- 2-Chloro-6-((2-chloroethyl)thio)-4-(trifluoromethyl)pyridine (CAS 1053657-72-9)
- Ethoate-methyl (CAS 116-01-8)
- Abiraterone Impurity 12 (CAS CATO-C4X-107012)
- 4-(prop-2-yn-1-yl)oxan-4-ol (CAS 1258641-47-2)
- 2-Chloro-5-(2,4-dichlorophenyl)isonicotinic acid (CAS 1261899-58-4)
- 4-(Pyridin-3-yl)-4,5-dihydropyrrolo[1,2-a]quinoxaline (CAS 358721-70-7)
- Tirzepatide Impurity 17 (CAS CATO-C4X-324017)
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