3-Benzyl-5-((2-fluoro-4-nitrophenyl)amino)-8-methylpyrido[2,3-d]pyrimidine-4,7(3H,8H)-dione CAS 1035556-25-2
| CAS No. | 1035556-25-2 |
| Molecular Formula | C21H16FN5O4 |
| Molecular Weight | 421.38 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
CATO supplies 3-Benzyl-5-((2-fluoro-4-nitrophenyl)amino)-8-methylpyrido[2,3-d]pyrimidine-4,7(3H,8H)-dione (CAS 1035556-25-2), a certified reference material (molecular formula C21H16FN5O4, MW 421.38 g/mol) for analytical method development and quality control.
The molecular framework of 3-Benzyl-5-((2-fluoro-4-nitrophenyl)amino)-8-methylpyrido[2,3-d]pyrimidine-4,7(3H,8H)-dione (C21H16FN5O4) is characterized by a flavonoid core, an amide group, a hydroxyl group, an ether linkage, fluorine substitution. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
Physical & Chemical Properties
| Molecular Formula | C21H16FN5O4 |
| Molecular Weight | 421.38 g/mol |
| Appearance | Pale yellow to yellow crystalline powder. (slight halide-like odor) |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening; Contains ester bond; hydrolyzes under strong alkaline conditions |
| Degree of Unsaturation (DBE) | 16.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1988 |
| Halogen Content | F-containing |
| Nitrogen Atoms | 5 |
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Recommended Standards
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- 4-[4-(Pyridin-2-yl)pyrimidin-2-yl]phenyl 3-(trifluoromethyl)benzoate (CAS 477857-39-9)
- 2-({1-[(2,2-difluoro-2H-1,3-benzodioxol-5-yl)methyl]-2-oxo-1,2-dihydropyridin-3-yl}formamido)-2,3-dimethylbutanamide (CAS 956181-54-7)
- N-((1-Ethyl-3-methyl-1H-pyrazol-4-yl)methyl)-1-isopropyl-1H-pyrazol-5-amine hydrochloride (CAS 1856044-22-8)
- Ethyl 2-(2,4-dichlorophenyl)thiazole-4-carboxylate (CAS 1185155-89-8)
- Ethyl 2-amino-4-(3-fluorophenyl)thiophene-3-carboxylate (CAS 849659-38-7)
- n-Methyl-2-(1,3,5-trimethyl-1h-pyrazol-4-yl)ethan-1-amine (CAS 956950-96-2)
- N-(4-methoxyphenyl)-1-(1-methyl-2-(3-(trifluoromethyl)phenoxy)-1H-indol-3-yl)methanimine (CAS 866017-46-1)
- 2-(4-Methoxybenzyl)-3,4-dihydro-2,6-naphthyridin-1(2H)-one (CAS 1365762-81-7)
- 3-Methoxy-4-[[4-(trifluoromethyl)phenyl]amino]cyclobut-3-ene-1,2-dione (CAS 1132827-67-8)
- tert-Butyl (3-hydroxy-1-phenylpropyl)carbamate (CAS 218449-48-0)
- 18-Hydroxyoctadecanoic acid (CAS 3155-42-8)
- (Leu31,Pro34)-Peptide YY (human) trifluoroacetate salt (CAS 179986-95-9)
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