25-Hydroxy Cholesterol CAS 2140-46-7

Purity >95%In StockPharmaceutical Standards
CAS No.2140-46-7
Molecular FormulaC27H46O2
Molecular Weight402.65 g/mol
CategoryPharmaceutical Standards / API Standards
AvailabilityIn Stock

Chemical Structure

25-Hydroxy Cholesterol CAS 2140-46-7 chemical structure

Description

Compared with other compounds in its class, the molecular weight of 402.65 g/mol gives 25-Hydroxy Cholesterol distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

25-Hydroxy Cholesterol (CAS 2140-46-7) is a high-purity analytical reference standard with the molecular formula C27H46O2 and a molecular weight of 402.65 g/mol.

Based on its molecular formula C27H46O2, 25-Hydroxy Cholesterol contains a steroid core, a hydroxyl group, an ether linkage; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 402.65 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. The high carbon-to-hydrogen ratio imparts good lipophilicity, allowing liquid-liquid extraction with non-polar solvents such as n-hexane or ethyl acetate.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

CATO manufactures 25-Hydroxy Cholesterol under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.

Physical & Chemical Properties

Molecular FormulaC27H46O2
Molecular Weight402.65 g/mol
AppearanceOff-white to pale yellow crystalline powder
Purity>95%
StorageStore at room temperature
Solubility (reference)Soluble in chloroform, dichloromethane, ethyl acetate, DMSO
StabilityMay oxidize slowly on prolonged air exposure
Degree of Unsaturation (DBE)5.0
UV AbsorptionUV-absorbing
Structural FeaturesAromatic ring present
CAS Registry Year (est.)1957

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