Cyanocobalamin EP Impurity A CAS 23208-66-4

Purity >95%In StockPharmacopoeial Impurity Standards
CAS No.23208-66-4
Molecular FormulaC63H85CoN13O15P
Molecular Weight1354.37 g/mol
CategoryPharmacopoeial Impurity Standards / EP Impurities
AvailabilityIn Stock

Chemical Structure

Cyanocobalamin EP Impurity A CAS 23208-66-4 chemical structure

Description

Compared with other compounds in its class, the molecular weight of 1354.37 g/mol gives Cyanocobalamin EP Impurity A distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

As a process- or degradation-related impurity, Cyanocobalamin EP Impurity A (CAS 23208-66-4, C63H85CoN13O15P) is essential for impurity profiling, related-substances testing, and forced-degradation studies in pharmaceutical development.

Cyanocobalamin EP Impurity A (C63H85CoN13O15P) contains a nitrogen-containing heterocyclic scaffold, an amide group, a phosphate ester group, a hydroxyl group, an ether linkage. It is notably hygroscopic, the carboxyl group imparts weak acidity; these physicochemical properties determine the optimal preparation and storage of its standard solutions. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

Molecular FormulaC63H85CoN13O15P
Molecular Weight1354.37 g/mol
Purity>95%
StorageStore in a cool place (≤25°C), protect from light, keep sealed and dry
Solubility (reference)Soluble in DMSO, DMF, methanol
StabilityMay oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening
Degree of Unsaturation (DBE)28.0
UV AbsorptionUV-absorbing
Structural FeaturesAromatic ring present
CAS Registry Year (est.)1963
Nitrogen Atoms13

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