(4Z)-4-({[(4-chlorophenyl)methyl]amino}methylidene)-3-methyl-1-phenyl-4,5-dihydro-1H-pyrazol-5-one CAS 320425-10-3

Description

Recommended for analytical method development and validation; each product is supplied with a full Certificate of Analysis (COA) ensuring ISO 17034 metrological traceability.

(4Z)-4-({[(4-chlorophenyl)methyl]amino}methylidene)-3-methyl-1-phenyl-4,5-dihydro-1H-pyrazol-5-one (CAS 320425-10-3), molecular formula C18H16ClN3O and molecular weight 325.79 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of (4Z)-4-({[(4-chlorophenyl)methyl]amino}methylidene)-3-methyl-1-phenyl-4,5-dihydro-1H-pyrazol-5-one (C18H16ClN3O) is characterized by a steroid core, an amide group, a hydroxyl group, an ether linkage, chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by its high hydrophobicity (logP), which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Halogen substitution (F/Cl/Br/I) produces a distinctive isotope pattern in mass spectrometry, a valuable aid for qualitative confirmation.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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