Doxycycline EP Impurity B（HCl） CAS 3963-95-9
| CAS No. | 3963-95-9 |
| Molecular Formula | C22H22N2O8 |
| Molecular Weight | N/A |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.
Doxycycline EP Impurity B（HCl） (CAS 3963-95-9) is a certified pharmaceutical impurity reference standard with the molecular formula C22H22N2O8HCl and a molecular weight of 478.88 g/mol.
Doxycycline EP Impurity B（HCl） (C22H22N2O8HCl) contains a flavonoid core, an amide group, a hydroxyl group, an ether linkage, chlorine substitution. It is notably hygroscopic, halogen substitution raises its density and boiling point, the carboxyl group imparts weak acidity; these physicochemical properties determine the optimal preparation and storage of its standard solutions. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
As a certified reference material (CRM), Doxycycline EP Impurity B（HCl） meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
Physical & Chemical Properties
| Molecular Formula | C22H22N2O8 HCl |
| Molecular Weight | 478.88 g/mol |
| Appearance | Pale yellow to yellow crystalline powder. (slight halide-like odor) |
| Purity | >95% |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening; Contains ester bond; hydrolyzes under strong alkaline conditions |
| Degree of Unsaturation (DBE) | 12.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1958 |
| Halogen Content | Cl-containing |
| Nitrogen Atoms | 2 |
Related Doxycycline Standards
- Doxycycline Hydrochloride (CAS 10592-13-9)
- Doxycycline EP Impurity F (CAS 122861-53-4)
- Doxycycline Monohydrate (CAS 17086-28-1)
- Doxycycline-13CD3 (CAS 1902958-13-7)
- Doxycycline Impurity 20 (CAS 2000233-92-9)
- Doxycycline Hyclate (CAS 24390-14-5)
- Doxycycline-d3 (CAS 2747918-45-0)
- Doxycycline-d3 HCl (CAS 2747918-45-0 (free base))
Recommended Standards
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- {spiro[2.5]octan-6-yl}methanol (CAS 849671-56-3)
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- 5-methyl-2-(piperidin-4-yloxy)pyrimidine (CAS 1211584-35-8)
- 4-(Pyrrolidin-1-yl)-N-(4-(pyrrolidin-1-yl)benzyl)benzamide (CAS 1423037-21-1)
- 1-[5-(4-chlorophenyl)thiophen-2-yl]ethan-1-amine hydrochloride (CAS 1432681-86-1)
- 6-Hydroxy-3-methyl-5-phenyl-1-(p-tolyl)pyridazin-4(1H)-one (CAS 89314-17-0)
- Semaglutide Impurity 13 (CAS CATO-C4X-222613)
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