Fluorescein impurity 01 CAS 76579-17-4
| CAS No. | 76579-17-4 |
| Molecular Formula | C28H29ClN2O4 |
| Molecular Weight | 492.99 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including Fluorescein impurity 01.
CATO supplies Fluorescein impurity 01 (CAS 76579-17-4), a pharmacopoeial impurity reference standard (molecular formula C28H29ClN2O4, MW 492.99 g/mol) used for the identification and quantification of trace impurities in APIs.
Based on its molecular formula C28H29ClN2O4, Fluorescein impurity 01 contains a steroid core, an amide group, a hydroxyl group, an ether linkage, chlorine substitution; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 492.99 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.
CATO manufactures Fluorescein impurity 01 under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.
Physical & Chemical Properties
| Molecular Formula | C28H29ClN2O4 |
| Molecular Weight | 492.99 g/mol |
| Appearance | Pale yellow to yellow crystalline powder. (slight halide-like odor) |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening; Contains ester bond; hydrolyzes under strong alkaline conditions |
| Degree of Unsaturation (DBE) | 15.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1978 |
| Halogen Content | Cl-containing |
| Nitrogen Atoms | 2 |
Related Fluorescein Standards
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Recommended Standards
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- Ethyltri-n-propyltin (CAS 3440-79-7)
- 1-(2-Bromopropyl)-3-fluorobenzene (CAS 1021927-57-0)
- 1H-Isoindole (CAS 270-69-9)
- 4,4,5,5-Tetramethyl-2-(2,4,5-trimethylthiophen-3-yl)-1,3,2-dioxaborolane (CAS 2064117-97-9)
- Docosyl acetate (CAS 822-26-4)
- 3-(4-Chlorophenyl)-4-ethyl-5-(methylthio)-4H-1,2,4-triazole (CAS 31409-18-4)
- endo-Gly31-Semaglutide (CAS CATO-C4X-2226282)
Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
- Worldwide shipping with full export documentation (HS code guidance available)
- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com