Methotrexate Hydrate CAS 133073-73-1
| CAS No. | 133073-73-1 |
| Molecular Formula | C20H22N8O5 |
| Molecular Weight | N/A |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
Compared with other compounds in its class, the molecular weight of 472.46 g/mol gives Methotrexate Hydrate distinctive chromatographic separation conditions and mass-spectrometry parameter settings.
Methotrexate Hydrate (CAS 133073-73-1), molecular formula C20H22N8O5H2O and molecular weight 472.46 g/mol, is a CATO reference standard with documented purity and traceability.
Methotrexate Hydrate (C20H22N8O5H2O) contains a flavonoid core, an amide group, a hydroxyl group, an ether linkage. It is notably hygroscopic, the carboxyl group imparts weak acidity; these physicochemical properties determine the optimal preparation and storage of its standard solutions. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
As a certified reference material (CRM), Methotrexate Hydrate meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
Physical & Chemical Properties
| Molecular Formula | C20H22N8O5 H2O |
| Molecular Weight | 472.46 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | >95% |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 13.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1995 |
| Nitrogen Atoms | 8 |
Related Methotrexate Standards
- Methotrexate Impurity 41 (CAS 101810-76-8)
- Methotrexate Dihydrate (CAS 1212284-68-8)
- Methotrexate Hydrate EP Impurity B (CAS 1217459-90-9)
- Methotrexate Impurity 11 (CAS 143873-72-7)
- Methotrexate EP Impurity E (CAS 19741-14-1)
- Methotrexate EP Impurity C (CAS 2410-93-7)
- Methotrexate EP Impurity G (CAS 2519848-42-9)
- Methotrexate EP Impurity G Sodium Salt (CAS 2519848-42-9（free base）)
Recommended Standards
- 3-(4-Aminophenyl)-N,N-dimethylpropanamide (CAS 1018284-46-2)
- 2-(1-(2-Methoxyethyl)-2,5-dimethyl-1H-pyrrol-3-yl)-2-oxoethyl 2-(methylthio)nicotinate (CAS 852545-48-3)
- (3,5-Difluorophenyl)(4-pentylphenyl)methanone (CAS 951887-69-7)
- 2-Nitronicotinaldehyde (CAS 5175-26-8)
- 2-Chloro-1-(1-(2-nitrobenzyl)-1H-pyrrol-2-yl)ethanone (CAS 81729-46-6)
- 1,1,2,2,3,4,4-octafluoro-1-iodobutane (CAS CATO-C3D-262157)
- 4-(4-Chlorophenoxy)-3-methylaniline (CAS 857007-14-8)
- 2-Ethyl-6-hexanesulfonyl chloride (CAS 62371-24-8)
- N-Benzyl-1-(trimethylsilyl)-N-((trimethylsilyl)methyl)methanamine (CAS 144964-17-0)
- Peptide Impurity 28 (CAS 1350456-64-2)
Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
- Worldwide shipping with full export documentation (HS code guidance available)
- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com