Methotrexate EP Impurity C CAS 2410-93-7
| CAS No. | 2410-93-7 |
| Molecular Formula | C20H21N7O6 |
| Molecular Weight | 455.42 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including Methotrexate EP Impurity C.
Methotrexate EP Impurity C (CAS 2410-93-7) is a certified pharmaceutical impurity reference standard with the molecular formula C20H21N7O6 and a molecular weight of 455.42 g/mol.
Structurally, Methotrexate EP Impurity C (C20H21N7O6) contains a flavonoid core, an amide group, a hydroxyl group, an ether linkage. The molecule exhibits a degree of unsaturation (DBE) of 14.0, corresponding to approximately 14 ring(s) or double-bond equivalent(s). With a molecular weight of 455.42 g/mol, it falls into the moderately-high-molecular-weight range. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C20H21N7O6 |
| Molecular Weight | 455.42 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | >95% |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 14.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1957 |
| Nitrogen Atoms | 7 |
Related Methotrexate Standards
- Methotrexate EP Impurity F (CAS 51865-79-3)
- Methotrexate Impurity 64 (CAS 52980-66-2)
- Methotrexate (CAS 59-05-2)
- Methotrexate Impurity 2 (CAS 80407-56-3)
- Methotrexate-d3 (CAS 432545-63-6)
- Methotrexate EP Impurity H (CAS 67022-39-3)
- Methotrexate EP Impurity I (CAS 66147-29-3)
- Methotrexate Impurity 11 (CAS 143873-72-7)
Recommended Standards
- Ninopterin (CAS 2179-16-0)
- PF-04457845 (CAS 1020315-31-4)
- Methyl 3-nitro-4-{4-[(3-oxo-3,4-dihydro-2H-1,4-benzoxazin-6-yl)carbamoyl]piperazin-1-yl}benzoate (CAS 866137-44-2)
- (R)-3-((((9h-Fluoren-9-yl)methoxy)carbonyl)amino)-4-(2,4,5-trifluorophenyl)butanoic acid (CAS 1217818-53-5)
- Linagliptin Impurity 113 (CAS 2803369-89-1)
- Solriamfetol Impurity 23 (CAS CATO-C4X-256323)
- CAS 2756307-47-6 (CAS 2756307-47-6)
- 21-Deacetoxy 11-Oxodeflazacort (CAS 13649-83-7)
- 1-Methyl-4-(2-(4-nitrophenyl)hydrazono)piperidine (CAS 75912-39-9)
- Bromoacetonitrile (CAS 590-17-0)
- Trans-O-Coumaric Acid (CAS 614-60-8)
- 2,2'-((2-((Carboxy(2-(16,16-dichlorohexadecyl)phenyl)methyl)(carboxymethyl)amino)ethyl)azanediyl)diacetic acid (CAS 41640-86-2)
- 2-(2-Bromo-4-chlorophenyl)-1,3-dioxolane (CAS 2221812-34-4)
- Glucagon Monohydrochloride (CAS 28270-04-4)
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