Methotrexate Hydrate EP Impurity B CAS 1217459-90-9
| CAS No. | 1217459-90-9 |
| Molecular Formula | C19H20N8O5 |
| Molecular Weight | N/A |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
Based on its molecular formula C19H20N8O5H2O, Methotrexate Hydrate EP Impurity B contains a flavonoid core, an amide group, a hydroxyl group, an ether linkage; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 458.43 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
Methotrexate Hydrate EP Impurity B (CAS 1217459-90-9) is a certified pharmaceutical impurity reference standard with the molecular formula C19H20N8O5H2O and a molecular weight of 458.43 g/mol.
Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.
CATO manufactures Methotrexate Hydrate EP Impurity B under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.
Physical & Chemical Properties
| Molecular Formula | C19H20N8O5 H2O |
| Molecular Weight | 458.43 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 13.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1990 |
| Nitrogen Atoms | 8 |
Related Methotrexate Standards
- Methotrexate Impurity 41 (CAS 101810-76-8)
- Methotrexate Dihydrate (CAS 1212284-68-8)
- Methotrexate Hydrate (CAS 133073-73-1)
- Methotrexate Impurity 11 (CAS 143873-72-7)
- Methotrexate EP Impurity E (CAS 19741-14-1)
- Methotrexate EP Impurity C (CAS 2410-93-7)
- Methotrexate EP Impurity G (CAS 2519848-42-9)
- Methotrexate EP Impurity G Sodium Salt (CAS 2519848-42-9（free base）)
Recommended Standards
- Paliperidone Impurity 58 (CAS CATO-C4X-172458)
- (6R,7S)-Ceftriaxone Impurity (CAS CATO-C4X-116316)
- Terbutaline EP Impurity A (CAS 99-10-5)
- N,N'-methylenediacetamide (CAS 3852-14-0)
- Medroxyprogesterone impurity 11 (CAS 2128317-86-0)
- N'-(4-Chlorobenzylidene)-5-(1-methyl-1H-pyrrol-2-yl)-1H-pyrazole-3-carbohydrazide (CAS 303106-80-1)
- Homosibutramine (CAS 935888-80-5)
- 4-(Furan-2-yl)thiophene-2-carboxylic acid (CAS 893727-89-4)
- 3-methoxypyrrolidine;hydrochloride (CAS 136725-50-3)
- Sperm Peptide P10G (H-2424.0001) (CAS 137353-73-2)
Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
- Worldwide shipping with full export documentation (HS code guidance available)
- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com