Riociguat Impurity N49 CAS 256376-59-7

Purity 97%+In StockPharmacopoeial Impurity Standards
CAS No.256376-59-7
Molecular FormulaC16H14FN3O2
Molecular Weight299.30 g/mol
CategoryPharmacopoeial Impurity Standards / EP Impurities
AvailabilityIn Stock

Chemical Structure

Riociguat Impurity N49 CAS 256376-59-7 chemical structure

Description

Compared with other compounds in its class, the molecular weight of 299.30 g/mol gives Riociguat Impurity N49 distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

CATO supplies Riociguat Impurity N49 (CAS 256376-59-7), a pharmacopoeial impurity reference standard (molecular formula C16H14FN3O2, MW 299.30 g/mol) used for the identification and quantification of trace impurities in APIs.

Riociguat Impurity N49 (C16H14FN3O2) contains a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage, fluorine substitution. It is notably hygroscopic, halogen substitution raises its density and boiling point, the carboxyl group imparts weak acidity; these physicochemical properties determine the optimal preparation and storage of its standard solutions. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.

Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.

As a certified reference material (CRM), Riociguat Impurity N49 meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

Molecular FormulaC16H14FN3O2
Molecular Weight299.30 g/mol
AppearancePale yellow to yellow crystalline powder. (slight halide-like odor)
Purity97%+
StorageStore at 2-8°C, protect from light, keep sealed and dry, keep tightly closed to prevent evaporation
Solubility (reference)Soluble in DMSO, DMF, methanol
StabilityMay oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening; Contains ester bond; hydrolyzes under strong alkaline conditions
Degree of Unsaturation (DBE)11.0
UV AbsorptionUV-absorbing
Structural FeaturesAromatic ring present
CAS Registry Year (est.)2025
Halogen ContentF-containing
Nitrogen Atoms3

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