Methotrexate-d3 CAS 432545-63-6
| CAS No. | 432545-63-6 |
| Molecular Formula | - |
| Molecular Weight | 457.46 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
The molecular framework of Methotrexate-d3 (C20H19D3N8O5) is characterized by deuterium isotope labeling, a flavonoid core, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
Methotrexate-d3 (CAS 432545-63-6) is a high-purity analytical reference standard with the molecular formula C20H19D3N8O5 and a molecular weight of 457.46 g/mol.
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
CATO manufactures Methotrexate-d3 under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.
Physical & Chemical Properties
| Molecular Formula | C20H19D3N8O5 |
| Molecular Weight | 457.46 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | >95% |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 15.5 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 2025 |
| Nitrogen Atoms | 8 |
Related Methotrexate Standards
- Methotrexate EP Impurity C (CAS 2410-93-7)
- Methotrexate EP Impurity F (CAS 51865-79-3)
- Methotrexate Impurity 64 (CAS 52980-66-2)
- Methotrexate (CAS 59-05-2)
- Methotrexate Impurity 2 (CAS 80407-56-3)
- Methotrexate EP Impurity H (CAS 67022-39-3)
- Methotrexate EP Impurity I (CAS 66147-29-3)
- Methotrexate Impurity 28 (CAS 5939-37-7)
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Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
- Worldwide shipping with full export documentation (HS code guidance available)
- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com