Methotrexate EP Impurity I CAS 66147-29-3
| CAS No. | 66147-29-3 |
| Molecular Formula | C21H24N8O5 |
| Molecular Weight | 468.47 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
Compared with other compounds in its class, the molecular weight of 468.47 g/mol gives Methotrexate EP Impurity I distinctive chromatographic separation conditions and mass-spectrometry parameter settings.
As a process- or degradation-related impurity, Methotrexate EP Impurity I (CAS 66147-29-3, C21H24N8O5) is essential for impurity profiling, related-substances testing, and forced-degradation studies in pharmaceutical development.
Based on its molecular formula C21H24N8O5, Methotrexate EP Impurity I contains a flavonoid core, an amide group, a hydroxyl group, an ether linkage; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 468.47 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).
Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C21H24N8O5 |
| Molecular Weight | 468.47 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | >95% |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 14.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1975 |
| Nitrogen Atoms | 8 |
Related Methotrexate Standards
- Methotrexate EP Impurity H (CAS 67022-39-3)
- Methotrexate Impurity 28 (CAS 5939-37-7)
- Methotrexate-d3 (CAS 432545-63-6)
- Methotrexate Impurity 18 (CAS CATO-C4X-115418)
- Methotrexate Dimethylamide (CAS 71074-44-7)
- Methotrexate EP Impurity C (CAS 2410-93-7)
- Methotrexate impurity N3 (CAS 65148-64-3)
- Methotrexate EP Impurity J (CAS 34378-65-9)
Recommended Standards
- Fmoc-Gly-Gly-Gly-Gly-OH (CAS 1001202-16-9)
- N-(5-((2-Oxo-2-((4-(trifluoromethyl)phenyl)amino)ethyl)thio)-1,3,4-thiadiazol-2-yl)-2-phenoxyacetamide (CAS 897757-87-8)
- 1,2-Bis[2-methylbenzo[b]thiophen-3-yl]-3,3,4,4,5,5-hexafluoro-1-cyclopentene (CAS 137814-07-4)
- N-(5-((2-Oxo-2-((3-(trifluoromethyl)phenyl)amino)ethyl)thio)-1,3,4-thiadiazol-2-yl)-2-phenoxyacetamide (CAS 897620-55-2)
- Gimeracil Impurity 1 (CAS CATO-C4X-12141)
- Landiolol Impurity 128 (CAS CATO-C4X-2621128)
- Relugoli Impurity 145 (CAS CATO-C4X-2429145)
- 4-[(E)-[(5-Bromo-2-methoxyphenyl)methylidene]amino]phenyl 2,4,6-trimethylbenzene-1-sulfonate (CAS 329777-90-4)
- (S)-(-)-PH-797804 (CAS 1358027-80-1)
- Methyl 3-amino-4-methoxythieno[2,3-b]pyridine-2-carboxylate (CAS 208994-11-0)
- 4-(1,3,4-thiadiazol-2-yl)aniline (CAS 875237-02-8)
- 2H-spiro[1-benzofuran-3,1'-cyclohexan]-4'-one (CAS 179064-52-9)
- dl-3-Methylvaline, HCl (CAS 93667-66-4)
- C-Peptide (human) (CAS 33017-11-7)
Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
- Worldwide shipping with full export documentation (HS code guidance available)
- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com