Methotrexate EP Impurity F CAS 51865-79-3
| CAS No. | 51865-79-3 |
| Molecular Formula | C20H22N8O5 |
| Molecular Weight | 454.44 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
CATO supplies Methotrexate EP Impurity F (CAS 51865-79-3), a pharmacopoeial impurity reference standard (molecular formula C20H22N8O5, MW 454.44 g/mol) used for the identification and quantification of trace impurities in APIs.
The molecular framework of Methotrexate EP Impurity F (C20H22N8O5) is characterized by a flavonoid core, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.
CATO manufactures Methotrexate EP Impurity F under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.
Physical & Chemical Properties
| Molecular Formula | C20H22N8O5 |
| Molecular Weight | 454.44 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | >90% |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 14.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1971 |
| Nitrogen Atoms | 8 |
Related Methotrexate Standards
- Methotrexate Impurity 64 (CAS 52980-66-2)
- Methotrexate (CAS 59-05-2)
- Methotrexate Impurity 2 (CAS 80407-56-3)
- Methotrexate EP Impurity C (CAS 2410-93-7)
- Methotrexate-d3 (CAS 432545-63-6)
- Methotrexate Impurity 11 (CAS 143873-72-7)
- Methotrexate EP Impurity B (CAS 54-62-6)
- Methotrexate EP Impurity H (CAS 67022-39-3)
Recommended Standards
- (+/-)Amethopterin (CAS 60388-53-6)
- 1,4-Bis(2-(benzo[d][1,3]dioxol-5-ylmethylene)hydrazinyl)phthalazine (CAS 27704-20-7)
- 1-(3-Methoxybenzyl)-3-methyl-4-(3-(trifluoromethyl)benzoyl)-3,4-dihydroquinoxalin-2(1H)-one (CAS 317833-51-5)
- 2,5-Dichloro-N-(4-(4-pentylcyclohexyl)phenyl)benzenesulfonamide (CAS 478041-42-8)
- Dronedarone Impurity 90 (CAS CATO-C4X-109290)
- Atosiban Impurity 60 (CAS CATO-C4X-229360)
- Alectinib Impurity 48 (CAS CATO-C4X-283648)
- 2-(2-Aminoquinolin-7-yl)acetic acid (CAS 1146298-72-7)
- Flomoxef (CAS 99665-00-6)
- 1-Iodo-3-methyl-2-(trifluoromethyl)benzene (CAS 1369955-63-4)
- 1-(4-Bromophenyl)-3-(chloromethyl)-1H-pyrazole (CAS 1468759-86-5)
- 4-(3-Bromothiophene-2-carboxamido)pentanoic acid (CAS 1340033-22-8)
- 3-Bromo-2-(3,3-difluoropiperidin-1-yl)nitrobenzene (CAS 1713163-26-8)
- Semaglutide impurity 198 (CAS CATO-C4X-2226198)
Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
- Worldwide shipping with full export documentation (HS code guidance available)
- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com