Methotrexate Impurity 64 CAS 52980-66-2
| CAS No. | 52980-66-2 |
| Molecular Formula | C20H22N8O5 |
| Molecular Weight | 454.44 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
This reference standard supports related-substances testing and method development in line with ICH Q3A/Q3B guidance on impurity control, and is suitable for system suitability testing and forced-degradation studies.
As a process- or degradation-related impurity, Methotrexate Impurity 64 (CAS 52980-66-2, C20H22N8O5) is essential for impurity profiling, related-substances testing, and forced-degradation studies in pharmaceutical development.
Owing to a flavonoid core, an amide group, a hydroxyl group, an ether linkage, Methotrexate Impurity 64 is typically pale yellow to yellow crystalline powder. Its solid-state physicochemical attributes — including a molecular weight of 454.44 g/mol — guide the selection of appropriate sample preparation and analytical techniques. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
CATO manufactures Methotrexate Impurity 64 under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.
Physical & Chemical Properties
| Molecular Formula | C20H22N8O5 |
| Molecular Weight | 454.44 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 14.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1972 |
| Nitrogen Atoms | 8 |
Related Methotrexate Standards
- Methotrexate EP Impurity F (CAS 51865-79-3)
- Methotrexate (CAS 59-05-2)
- Methotrexate Impurity 2 (CAS 80407-56-3)
- Methotrexate EP Impurity C (CAS 2410-93-7)
- Methotrexate-d3 (CAS 432545-63-6)
- Methotrexate Impurity 11 (CAS 143873-72-7)
- Methotrexate EP Impurity B (CAS 54-62-6)
- Methotrexate EP Impurity H (CAS 67022-39-3)
Recommended Standards
- (+/-)Amethopterin (CAS 60388-53-6)
- 2,5-Dichloro-N-(4-(4-pentylcyclohexyl)phenyl)benzenesulfonamide (CAS 478041-42-8)
- 1,4-Bis(2-(benzo[d][1,3]dioxol-5-ylmethylene)hydrazinyl)phthalazine (CAS 27704-20-7)
- GW779439X (CAS 551919-98-3)
- Amphotericin B Impurity 18 (CAS CATO-C4X-184418)
- Artesunate Impurity 6 (CAS CATO-C4X-15476)
- Choline fenofibrate Impurity 23 (CAS 154356-97-5)
- Methyl 8-chloro-2-methyl-4-oxo-3,4,5,6-tetrahydro-2H-2,6-methanobenzo[g][1,3]oxazocine-5-carboxylate (CAS 353296-61-4)
- 2',3',4,4',5',6'-Hexafluoro-[1,1'-biphenyl]-2-carboxylic acid (CAS 1261836-24-1)
- 2-amino-2-(1H-1,2,3,4-tetrazol-5-yl)acetic acid (CAS 138199-51-6)
- Voglibose Impurity 27 (CAS CATO-C4X-178027)
- 1,4-Phenylenebis((4-(4-aminophenoxy)phenyl)methanone) (CAS 107194-50-3)
- 3-Chloro-6-hydrazinylpyridazine hydrochloride (CAS 856847-88-6)
- Ephrin-A2-Selective YSA-Peptide (CAS 532441-10-4)
Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
- Worldwide shipping with full export documentation (HS code guidance available)
- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com